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Molecularly Imprinted Polymers Exhibit Low Cytotoxic and Inflammatory Properties in Macrophages In Vitro
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces. Phase Holographic Imaging AB, SE-223 63 Lund, Sweden.
Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.
Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.ORCID iD: 0000-0002-2043-4522
Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.ORCID iD: 0000-0002-5589-5548
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2022 (English)In: Applied Sciences, E-ISSN 2076-3417, Vol. 12, p. 1-16, article id 6091Article in journal (Refereed) Published
Abstract [en]

Molecularly imprinted polymers (MIPs) against sialic acid (SA) have been developed as a detection tool to target cancer cells. Before proceeding to in vivo studies, a better knowledge of the overall effects of MIPs on the innate immune system is needed. The aim of this study thus was to exemplarily assess whether SA-MIPs lead to inflammatory and/or cytotoxic responses when administered to phagocytosing cells in the innate immune system. The response of monocytic/macrophage cell lines to two different reference particles, Alhydrogel and PLGA, was compared to their response to SA-MIPs. In vitro culture showed a cellular association of SA-MIPs and Alhydrogel, as analyzed by flow cytometry. The reference particle Alhydrogel induced secretion of IL-1β from the monocytic cell line THP-1, whereas almost no secretion was provoked for SA-MIPs. A reduced number of both THP-1 and RAW 264.7 cells were observed after incubation with SA-MIPs and this was not caused by cytotoxicity. Digital holographic cytometry showed that SA-MIP treatment affected cell division, with much fewer cells dividing. Thus, the reduced number of cells after SA-MIP treatment was not linked to SA-MIPs cytotoxicity. In conclusion, SA-MIPs have a low degree of inflammatory properties, are not cytotoxic, and can be applicable for future in vivo studies.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 12, p. 1-16, article id 6091
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:mau:diva-53523DOI: 10.3390/app12126091ISI: 000818495900001Scopus ID: 2-s2.0-85132751550OAI: oai:DiVA.org:mau-53523DiVA, id: diva2:1676286
Available from: 2022-06-24 Created: 2022-06-24 Last updated: 2023-10-02Bibliographically approved
In thesis
1. Connecting sialic acid expression to cancer cell characteristics: Novel tools for detection, imaging, and analysis
Open this publication in new window or tab >>Connecting sialic acid expression to cancer cell characteristics: Novel tools for detection, imaging, and analysis
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sialic acid (SA) plays a crucial role in many biological processes. Cell surface SA expression is usually analyzed with antibodies or lectins; however, they are costly and with poor stability. We have used a molecular imprinting technique to synthesize an alternative SA receptor – SA molecularly imprinted polymers(SA-MIPs) with an embedded fluorophore for fluorescent detection of theSA-MIPs. The binding behavior and specificity of SA-MIPs were verified by using lectins and SA conjugates on cancer cell lines, showing that SA-MIPs can be used as an effective tool for SA expression analysis of cancer cells. Digital holographic cytometry (DHC) is a non-phototoxic quantitative phase imaging technique that facilitates the monitoring of living cells over time. We have demonstrated the potential of DHC by mapping cellular parameters, such as cell number, area, thickness, and volume. In addition, cellular parameters possibly depending on sialylation, were evaluated using DHC. Furthermore, the uptake over time of SA-MIPs by macrophages was investigated for any inflammatory and/or cytotoxic responses when administered to phagocytosing cells. Our results indicate that SA-MIPs caused low induction and sparse secretion of inflammatory cytokines, and that reduced cell proliferation was not due to cytotoxicity, but to attenuated cell cycles. These results suggest that SA-MIPs will contribute to the further understanding of cancer cell behavior and can be an asset for in vivo studies.

Place, publisher, year, edition, pages
Malmö: Malmö University Press, 2022. p. 83
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 2022:9
Keywords
Molecularly imprinted polymers, digital holographic cytometry, cancer, cytotoxicity, inflammation, sialic acid
National Category
Medical and Health Sciences
Research subject
Health and society
Identifiers
urn:nbn:se:mau:diva-56210 (URN)10.24834/isbn.9789178773251 (DOI)978-91-7877-326-8 (ISBN)9789178773251 (ISBN)
Public defence
2022-12-15, AS:E002, Malmö, 10:12 (English)
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Note

Incorrect ISBN in publication: 978-91-7877-326-1 (pdf)

Paper II in dissertation as manuscript and is not included in the fulltext online 

Available from: 2022-11-25 Created: 2022-11-25 Last updated: 2024-03-07Bibliographically approved

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Sternbæk, LouiseGjörloff Wingren, AnetteEriksson, Håkan

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Sternbæk, LouiseGawlitza, KorneliaRurack, KnutAlm, KerstiGjörloff Wingren, AnetteEriksson, Håkan
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