Malmö University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Glucose-to-Resistor Transduction Integrated into a Radio-Frequency Antenna for Chip-less and Battery-less Wireless Sensing
Malmö University, Biofilms Research Center for Biointerfaces. Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Department of Biomedical Science, Faculty of Health and Society, Malmö University, Malmö 205 06, Sweden;Biofilms−Research Center for Biointerfaces, Malmö University, Malmö 205 06, Sweden.
State Research Institute, Centre for Physical Sciences and Technology, Saulėtekio av. 3, Vilnius LT-10257, Lithuania.
Institute of Biochemistry, Life Sciences Centre, Vilnius University, Saulėtekio al. 7, Vilnius LT-10223, Lithuania;Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Saulėtekio al. 11, Vilnius LT-10223, Lithuania.ORCID iD: 0000-0003-3938-3574
Institute of Biochemistry, Life Sciences Centre, Vilnius University, Saulėtekio al. 7, Vilnius LT-10223, Lithuania.
Show others and affiliations
2022 (English)In: ACS Sensors, E-ISSN 2379-3694, Vol. 7, no 4, p. 1222-1234Article in journal (Refereed) Published
Abstract [en]

To maximize the potential of 5G infrastructure in healthcare, simple integration of biosensors with wireless tag antennas would be beneficial. This work introduces novel glucose-to-resistor transduction, which enables simple, wireless biosensor design. The biosensor was realized on a near-field communication tag antenna, where a sensing bioanode generated electrical current and electroreduced a nonconducting antenna material into an excellent conductor. For this, a part of the antenna was replaced by a Ag nanoparticle layer oxidized to high-resistance AgCl. The bioanode was based on Au nanoparticle-wired glucose dehydrogenase (GDH). The exposure of the cathode-bioanode to glucose solution resulted in GDH-catalyzed oxidation of glucose at the bioanode with a concomitant reduction of AgCl to highly conducting Ag on the cathode. The AgCl-to-Ag conversion strongly affected the impedance of the antenna circuit, allowing wireless detection of glucose. Mimicking the final application, the proposed wireless biosensor was ultimately evaluated through the measurement of glucose in whole blood, showing good agreement with the values obtained with a commercially available glucometer. This work, for the first time, demonstrates that making a part of the antenna from the AgCl layer allows achieving simple, chip-less, and battery-less wireless sensing of enzyme-catalyzed reduction reaction. 

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2022. Vol. 7, no 4, p. 1222-1234
Keywords [en]
Internet of Things, wireless detection of glucose, direct electron transfer, glucose dehydrogenase, chip-less wireless sensing
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:mau:diva-51019DOI: 10.1021/acssensors.2c00394ISI: 000794994500032PubMedID: 35392657Scopus ID: 2-s2.0-85128799436OAI: oai:DiVA.org:mau-51019DiVA, id: diva2:1650841
Funder
Swedish Research Council, 2018-04320Knowledge Foundation, 20170058Knowledge Foundation, 20190010Available from: 2022-04-08 Created: 2022-04-08 Last updated: 2024-03-05Bibliographically approved
In thesis
1. Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
Open this publication in new window or tab >>Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Development of wireless sensors and biosensors is currently experiencing a rapid progress with a substantial focus directed toward highlighting their potential applications as non-invasive wearables, implants, and highly mobile point-of-care devices. Integration of wireless biosensors into the Internet of Things (IoT) is widely acknowledged as a technological advancement with the potential to significantly change daily life. To maximize this potential, simple integration of biosensors with wireless communication elements would be advantageous. In this regard, systems functioning in chipless, and battery-less modes outperform integrated circuit (IC) based and battery-powered wireless biosensors. Nevertheless, the accessibility of these wireless designs is still limited. In this study, we present a novel approach where incorporating silver nanoparticles(AgNPs) as a part of the radio frequency (RF) tag antenna enables the realization of simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions. We exemplified the mechanism of operation in such systems by electronic wiring of enzymes through direct electron transfer (DET) and microorganisms through mediated electron transfer (MET) to the redox conversion of Ag/AgCl. The wiring was designed to facilitate the transformation of metallic AgNPs into AgCl (Ag → AgCl) or the conversion of AgCl particles back into metallic AgNPs (AgCl → Ag) when the enzymatic/microorganism based electron transfer reactions were present. These reactions occurring on the biosensor RF tag antenna strongly changed the impedance of the tag, which was wirelessly monitored by a radio frequency identification (RFID) reader. The functionality of the proposed setup in direct electron transfer coupling of the enzymatic reactions to the redox conversion of the Ag/AgCl was demonstrated by wireless detection of glucose in whole blood samples and hydrogen peroxide penetrated through the skin membrane using the enzymes glucose dehydrogenase(GDH) and horseradish peroxidase (HRP). Additionally, the capability of the proposed configuration in mediated electron transfer wiring of microorganisms to the Ag/AgCl electrochemistry was shown by wireless monitoring of medically relevant microbial biofilms in simulated wound fluid. Generalizing, the results of this work, for the first time, demonstrated that exploiting Ag/AgCl as a part of the tag antenna allows simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions.

Place, publisher, year, edition, pages
Malmö: Malmö University Press, 2023. p. 108
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 99
National Category
Chemical Sciences Engineering and Technology Medical Biotechnology
Identifiers
urn:nbn:se:mau:diva-63289 (URN)10.24834/isbn.9789178774128 (DOI)9789178774111 (ISBN)9789178774128 (ISBN)
Public defence
2023-10-27, AS: E002, Faculty of Health and Society, 09:15
Opponent
Supervisors
Note

Paper IV in dissertation as manuscript.

Available from: 2023-10-31 Created: 2023-10-30 Last updated: 2024-02-27Bibliographically approved

Open Access in DiVA

fulltext(3247 kB)130 downloads
File information
File name FULLTEXT01.pdfFile size 3247 kBChecksum SHA-512
ebbaf1b2e34b6f83f69446b804003c4298488eb3e52f450b4caed5ff764e4bcdcf21ec7a1d0464be475d6aeef7f30d03f26fed40f90a765d11a140e13c85eba2
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Shafaat, AtefehGonzalez-Martinez, Juan FranciscoNeilands, JessicaBjörklund, SebastianSotres, JavierRuzgas, Tautgirdas

Search in DiVA

By author/editor
Shafaat, AtefehRatautas, DaliusGonzalez-Martinez, Juan FranciscoNeilands, JessicaBjörklund, SebastianSotres, JavierRuzgas, Tautgirdas
By organisation
Biofilms Research Center for BiointerfacesDepartment of Biomedical Science (BMV)Faculty of Odontology (OD)
In the same journal
ACS Sensors
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 130 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 212 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf