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Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B
Malmö University, Faculty of Odontology (OD).ORCID iD: 0000-0001-9545-9161
Malmö University, Faculty of Odontology (OD).ORCID iD: 0000-0003-3173-7577
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Lund Univ, Dept Immunotechnol, Lund, Sweden..
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2021 (English)In: Journal of Oral Microbiology, E-ISSN 2000-2297, Vol. 13, no 1, article id 1967636Article in journal (Refereed) Published
Abstract [en]

Background Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic 'mucolytic' activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology. Methods The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometry-based workflow, and differentially expressed proteins were identified. Results and conclusions Overall, the proteomic profiles of S. gordonii DL1 were similar across conditions. Six novel biofilm cell proteins and three planktonic proteins absent in all biofilm cultures were identified. These differences may provide insights into mechanisms for adaptation to biofilm growth in this species. Salivary MUC5B also elicited specific responses in the biofilm cell proteome. These regulations may represent mechanisms by which this mucin could promote colonization of the commensal S. gordonii in oral biofilms.

Place, publisher, year, edition, pages
Taylor & Francis, 2021. Vol. 13, no 1, article id 1967636
Keywords [en]
Salivary mucin, MUC5B, oral streptococci, Streptococcus gordonii, oral biofilm, saliva, mass spectrometry, protein expression, proteomics
National Category
Dentistry
Identifiers
URN: urn:nbn:se:mau:diva-45857DOI: 10.1080/20002297.2021.1967636ISI: 000687414300001PubMedID: 34447490Scopus ID: 2-s2.0-85120853173OAI: oai:DiVA.org:mau-45857DiVA, id: diva2:1593798
Available from: 2021-09-14 Created: 2021-09-14 Last updated: 2024-04-16Bibliographically approved
In thesis
1. Responses to External Cues in Oral Bacteria
Open this publication in new window or tab >>Responses to External Cues in Oral Bacteria
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis investigates responses to external cues in oral bacteria on a molecular level. Paper I maps Ser/Thr/Tyr phosphorylated proteins in relation to the general proteome in an oral commensal streptococcus (Streptococcus gordonii DL1). The identified phosphoproteins were involved in various bacterial processes, several associated to dysbiosis and development of biofilm-induced disease. Comparison against phosphoproteomes of other bacteria showed many similarities. This is of interest for the identification of shared phosphorylation profiles. 

Paper II studies differences between the S. gordonii DL1 general proteomes in planktonic and biofilm growth phases, and the regulatory effects of salivary mucin MUC5B on protein expression in the biofilm cells. Regulations in protein expression between the different growth conditions provides insights in bacterial mechanisms for adaptation to the biofilm lifestyle. 

Paper III examines the regulatory roles of salivary MUC5B on biofilm attachment and metabolic output in two clinical isolates of oral commensals, S. gordonii CW and Actinomyces naeslundii CW. S. gordonii facilitated adhesion of A. naeslundii to MUC5B during early attachment. Both bacteria were also able to utilize MUC5B as a sole nutrient source during early biofilm formation, individually and synergistically in a dual species biofilm. The specific responses elicited by MUC5B in paper II-III seem to promote commensal colonization while down-regulating dysbiosis-related biofilm activities. 

Microbiological studies are often focused on dysbiosis and development of disease. However, mechanisms that promote eubiosis are equally important to understand how health can be maintained. Findings associated with responses to external cues in oral bacteria may contribute to future development of novel preventative strategies and identification of predictive biomarkers for oral health. 

Abstract [sv]

Avhandlingen undersöker orala bakteriers svar till utifrån kommande signaler på en molekylärbiologisk nivå. I delarbete I kartläggs proteiner med serin/treonin/ tyrosin fosforylering i relation till det generella proteomet hos en oral streptokock (Streptococcus gordonii DL1). De identifierade fosfoproteinerna kunde kopplas till olika bakteriella processer, varav flera av intresse ur kariessynpunkt. Jämförelse mot andra bakteriers fosfoproteom visade många likheter, vilket är av intresse för identifiering av delade fosforyleringsprofiler. 

I delarbete II undersöks skillnader i det generella proteomet hos S. gordonii DL1 mellan tillväxtfaserna planktonisk och biofilm, samt reglerande effekter av salivmucinet MUC5B på biofilmscellerna proteinuttryck. Skillnaderna i proteinuttryck mellan tillväxtfaser ger ledtrådar om bakteriernas mekanismer för anpassning till biofilmstillväxt. 

Delarbete III studeras salivmucinet MUC5Bs reglerande roll på vidhäftning och metabolism i biofilmer med kliniska isolat av S. gordonii CW samt Actinomyces naeslundii CW. S. gordonii ökade vidhäftningen av A. naeslundii till MUC5B. Båda arterna kunde också använda MUC5B som enda näringskälla under tidig biofilmsbildning, både enskilt och tillsammans. Responserna som MUC5B framkallade i biofilmerna (paper II och III) verkar främja kolonisering av kommensaler och samtidigt nedreglera kariesrelaterade aktiviteter. 

Mikrobiologiska studier fokuserar ofta på dysbiosis och sjukdomsutveckling, men mekanismer som bibehåller eubiosis är minst lika viktiga för att förstå hur oral hälsa kan främjas. Resultat kopplade till orala bakteriers svar på utifrån kommande signaler kan bidra till framtida utveckling av nya strategier för prevention och identifiering av prediktiva biomarkörer för oral hälsa. 

Place, publisher, year, edition, pages
Malmö: Malmö University Press, 2023. p. 105
Series
Malmö University Odontological Dissertations, ISSN 1650-6065
Keywords
Oral Microbiology, Oral Biofilms, Saliva, Salivary mucin MUC5B, Streptococcus, Actinomyces
National Category
Microbiology Biochemistry and Molecular Biology Cell and Molecular Biology Dentistry
Identifiers
urn:nbn:se:mau:diva-62571 (URN)10.24834/isbn.9789178774180 (DOI)978-91-7877-417-3 (ISBN)978-91-7877-418-0 (ISBN)
Public defence
2023-12-08, KL:2370, Carl Gustafs väg 34, Malmö, 09:00 (English)
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Supervisors
Projects
Foresight
Funder
Swedish Research Council, 201601994NIH (National Institutes of Health), P005403501
Available from: 2023-10-27 Created: 2023-10-26 Last updated: 2024-02-23Bibliographically approved

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Robertsson, CarolinaSvensäter, GunnelBlum, ZoltanWickström, Claes

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