Publikationer från Malmö universitet
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Bacterial Composition and Metabolomics of Dental Plaque From Adolescents
Malmö universitet, Odontologiska fakulteten (OD). Department of Pediatric Dentistry, Institute for Postgraduate Dental Education, Jönköping, Sweden; Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden.
Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden; Folktandvården Skåne, The Swedish Dental Service of Skåne, Lund, Sweden.
Malmö universitet, Odontologiska fakulteten (OD). Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden; Folktandvården Skåne, The Swedish Dental Service of Skåne, Lund, Sweden.ORCID-id: 0000-0001-5145-8220
Department of Health & Genomics, Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation, Valencia, Spain.
Visa övriga samt affilieringar
2021 (Engelska)Ingår i: Frontiers in Cellular and Infection Microbiology, E-ISSN 2235-2988, Vol. 11, artikel-id 716493Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Supragingival dental plaque samples were collected from 40 Swedish adolescents, including 20 with caries lesions (CAR) and 20 caries-free (CF). Fresh plaque samples were subjected to an ex vivo acid tolerance (AT) test where the proportion of bacteria resistant to an acid shock was evaluated through confocal microscopy and live/dead staining, and the metabolites produced were quantified by 1H Nuclear Magnetic Resonance (1H NMR). In addition, DNA was extracted and the 16S rRNA gene was sequenced by Illumina sequencing, in order to characterize bacterial composition in the same samples. There were no significant differences in AT scores between CAR and CF individuals. However, 7 out of the 10 individuals with highest AT scores belonged to the CAR group. Regarding bacterial composition, Abiotrophia, Prevotella and Veillonella were found at significantly higher levels in CAR individuals (p=0.0085, 0.026 and 0.04 respectively) and Rothia and Corynebacterium at significantly higher levels in CF individuals (p=0.026 and 0.003). The caries pathogen Streptococcus mutans was found at low frequencies and was absent in 60% of CAR individuals. Random-forest predictive models indicate that at least 4 bacterial species or 9 genera are needed to distinguish CAR from CF adolescents. The metabolomic profile obtained by NMR showed a significant clustering of organic acids with specific bacteria in CAR and/or high AT individuals, being Scardovia wiggsiae the species with strongest associations. A significant clustering of ethanol and isopropanol with health-associated bacteria such as Rothia or Corynebacterium was also found. Accordingly, several relationships involving these compounds like the Ethanol : Lactate or Succinate : Lactate ratios were significantly associated to acid tolerance and could be of predictive value for caries risk. We therefore propose that future caries risk studies would benefit from considering not only the use of multiple organisms as potential microbial biomarkers, but also their functional adaptation and metabolic output.

Ort, förlag, år, upplaga, sidor
Frontiers Media S.A., 2021. Vol. 11, artikel-id 716493
Nyckelord [en]
NMR, Rothia, Streptococcus mutans (S. mutans), acid tolerance, caries risk (assessment), dental caries, metabolomics (OMICS), microbiome
Nationell ämneskategori
Odontologi
Identifikatorer
URN: urn:nbn:se:mau:diva-44983DOI: 10.3389/fcimb.2021.716493ISI: 000684641700001PubMedID: 34395316Scopus ID: 2-s2.0-85112476194OAI: oai:DiVA.org:mau-44983DiVA, id: diva2:1586493
Tillgänglig från: 2021-08-20 Skapad: 2021-08-20 Senast uppdaterad: 2024-02-05Bibliografiskt granskad

Open Access i DiVA

fulltext(2402 kB)123 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 2402 kBChecksumma SHA-512
8db6c7ee726654bd496cac795a983de0c0bff75873f1fb4ce24210f6819b0c99dd10777be27f1c6c737902619668445d04a8e899706cf9a8f4918cc68f4bd972
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Havsed, KristianJansson, HenrikNeilands, JessicaSvensäter, Gunnel

Sök vidare i DiVA

Av författaren/redaktören
Havsed, KristianJansson, HenrikNeilands, JessicaSvensäter, Gunnel
Av organisationen
Odontologiska fakulteten (OD)Biofilms Research Center for Biointerfaces
I samma tidskrift
Frontiers in Cellular and Infection Microbiology
Odontologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 123 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 100 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf