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Effect of nonionic and amphoteric surfactants on salivary pellicles reconstituted in vitro
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Rutherford Appleton Laboratory, UK.
Rutherford Appleton Laboratory, UK.
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2021 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 11, no 1, article id 12913Article in journal (Refereed) Published
Abstract [en]

Surfactants are important components of oral care products. Sodium dodecyl sulfate (SDS) is the most common because of its foaming properties, taste and low cost. However, the use of ionic surfactants, especially SDS, is related to several oral mucosa conditions. Thus, there is a high interest in using non-ionic and amphoteric surfactants as they are less irritant. To better understand the performance of these surfactants in oral care products, we investigated their interaction with salivary pellicles i.e., the proteinaceous films that cover surfaces exposed to saliva. Specifically, we focused on pentaethylene glycol monododecyl ether (C12E5) and cocamidopropyl betaine (CAPB) as model nonionic and amphoteric surfactants respectively, and investigated their interaction with reconstituted salivary pellicles with various surface techniques: Quartz Crystal Microbalance with Dissipation, Ellipsometry, Force Spectroscopy and Neutron Reflectometry. Both C12E5 and CAPB were gentler on pellicles than SDS, removing a lower amount. However, their interaction with pellicles differed. Our work indicates that CAPB would mainly interact with the mucin components of pellicles, leading to collapse and dehydration. In contrast, exposure to C12E5 had a minimal effect on the pellicles, mainly resulting in the replacement/solubilisation of some of the components anchoring pellicles to their substrate.

Place, publisher, year, edition, pages
Nature Publishing Group, 2021. Vol. 11, no 1, article id 12913
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:mau:diva-44076DOI: 10.1038/s41598-021-92505-4ISI: 000667261900001PubMedID: 34155330Scopus ID: 2-s2.0-85108451872OAI: oai:DiVA.org:mau-44076DiVA, id: diva2:1572041
Available from: 2021-06-23 Created: 2021-06-23 Last updated: 2024-02-05Bibliographically approved
In thesis
1. On the structure and mechanical properties of in vitro salivary pellicles
Open this publication in new window or tab >>On the structure and mechanical properties of in vitro salivary pellicles
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Salivary pellicles display exceptional hydration and lubrication performance. At present, there are still gaps in the understanding of how this is achieved. The aim of this thesis was therefore to increase our knowledge on the mechanisms underlying these properties and deepen the understanding of how they are related to the composition and structure of pellicles, with a focus on those formed under in vitro conditions. This has applications ranging from the development of artificial saliva and lubricating coatings for biomedical applications to methodological approaches for initial testing of oral healthcare products. For this, we also focused on developing suitable methodological approaches for these studies, centering on atomic force microscopy, quartz crystal microbalance with dissipation monitoring, ellipsometry and neutron reflectometry techniques, to investigate in vitro and model salivary pellicles.

First, we confirmed a two-layer structure for in vitro salivary pellicles and showed that the outer layer is mainly composed by the oral mucin MUC5B, but that it also contains other salivary components that enhance swelling and hydration. In the presence of bulk saliva, the outer layer also contains a reversibly and loosely bound fraction. This fraction increases the adhesiveness of the pellicle but unexpectedly has no significant effect on its lubrication performance. We also investigated the effect of mechanical confinement on model salivary pellicles by means of Neutron Reflectometry, revealing that at a pressure of 1 bar they are already completely compressed and dehydrated. Finally, with the aim to advance towards better oral healthcare products, we investigated the effect of nonionic and amphoteric surfactants on salivary pellicles, showing that they have a gentler effect on pellicle structure than the commonly employed anionic surfactants.

Place, publisher, year, edition, pages
Malmö universitet, 2021. p. 59
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 2022:1
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:mau:diva-49108 (URN)10.24834/isbn.9789178772414 (DOI)978-91-7877-240-7 (ISBN)978-91-7877-241-4 (ISBN)
Public defence
2022-01-21, Live stream and on location at HS assembly hall, Malmö, 10:00 (English)
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Supervisors
Note

Paper III in dissertation as manuscript.

Available from: 2022-01-10 Created: 2022-01-06 Last updated: 2024-01-09Bibliographically approved

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Boyd, HannahGonzalez-Martinez, Juan FArnebrant, ThomasSotres, Javier

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