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Optimering av Supported Liquid Extraction teknik vid LC-MS/MS analys av endogena steroider för klinisk diagnostik
Malmö University, Faculty of Health and Society (HS).
2021 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Vid upparbetning av endogena steroidhormon inför analys på LC-MS/MS används ofta Supported Liquid Extraction-teknik. Extraktionseffektiviteten beror av den interfas som bildas mellan vattenhaltigt prov och organiskt extraktionsmedel. Att använda lämpligt extraktionsmedel och metod är avgörande för att skapa förutsättningar för optimala analysförhållanden. Målsättningen att optimera extraktion med SLE-teknik testades genom att reducera volym extraktionsmedel samt utforska alternativa organiska lösningsmedel. Aldosteron, Androstendion, 17-hydroxiprogesteron och 11-deoxikortisol uppvisade med kriterier gällande regression och utbyte ingen betydelsefull variation mellan ordinarie referensmetod och testmetod med lägre extraktionsvolym. Studien kan därför potentiellt ligga till grund för vidare validering av den optimerade metoden. Det något mer känsliga steroidhormonet Östradiol uppvisade vid metodmodifikationer försämrat utbyte och kromatografi. Detta indikerar att vidare tester gällande typ och proportioner av extraktionsmedel lämpligen bör utföras.

Abstract [en]

Supported Liquid Extraction technology is often used in the preparation of endogenous steroid hormones prior to analysis on LC-MS/MS. The extraction efficiency depends on the interphase formed between the aqueous sample and the organic extractant. Using the appropriate type of extractant and method is crucial for creating optimal conditions for analysis. The aim of the study was to reduce the amount of chemicals and time spent on preparation by altering the volume and type of extractant. Aldosterone, Androstenedione, 17-hydroxyprogesterone and 11-deoxycortisol, with predetermined regression and exchange criteria in mind, showed no significant variation between the reference method and the test method with reduced extraction volume, which indicates that the study can be used as basis for further validation. The slightly more sensitive steroid hormone Estradiol showed impaired yield and chromatography as a result of method modifications. This indicates that further tests regarding the type and proportions of extractant should be performed appropriately. This indicates that further tests regarding the type and proportions of extractant could be relevant. 

Place, publisher, year, edition, pages
2021. , p. 33
Keywords [en]
Adrenal steroid hormones, Diatomaceous Earth, LC-MS/MS, Partitioning, Supported Liquid Extraction.
Keywords [sv]
Kiselgur, LC-MS/MS, Partitionering, Steroidhormon, Supported Liquid Extraction.
National Category
Clinical Laboratory Medicine Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:mau:diva-43216OAI: oai:DiVA.org:mau-43216DiVA, id: diva2:1564015
External cooperation
Region Skåne, Klinisk kemi och farmakologi, Specialkemi
Educational program
HS Biomedicinsk laboratorievetenskap
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Examiners
Available from: 2021-11-12 Created: 2021-06-10 Last updated: 2021-11-12Bibliographically approved

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