Publikationer från Malmö universitet
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Antimicrobial Potential of Strontium Hydroxide on Bacteria Associated with Peri-Implantitis
Malmö universitet, Odontologiska fakulteten (OD).
Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.ORCID-id: 0000-0003-3173-7577
Malmö universitet, Odontologiska fakulteten (OD). University of Geneva, Switzerland.ORCID-id: 0000-0001-8161-3754
2021 (Engelska)Ingår i: Antibiotics, ISSN 0066-4774, E-ISSN 2079-6382, Vol. 10, nr 2, artikel-id 150Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Peri-implantitis due to infection of dental implants is a common complication that may cause significant patient morbidity. In this study, we investigated the antimicrobial potential of Sr(OH)2 against different bacteria associated with peri-implantitis. Methods: The antimicrobial potential of five concentrations of Sr(OH)2 (100, 10, 1, 0.1, and 0.01 mM) was assessed with agar diffusion test, minimal inhibitory concentration (MIC), and biofilm viability assays against six bacteria commonly associated with biomaterial infections: Streptococcus mitis, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Escherichia coli, and Fusobacterium nucleatum. Results: Zones of inhibition were only observed for, 0.01, 0.1, and 1 mM of Sr(OH)2 tested against P. gingivalis, in the agar diffusion test. Growth inhibition in planktonic cultures was achieved at 10 mM for all species tested (p < 0.001). In biofilm viability assay, 10 and 100 mM Sr(OH)2 showed potent bactericidal affect against S. mitis, S. epidermidis, A. actinomycetemcomitans, E. coli, and P. gingivalis. Conclusions: The findings of this study indicate that Sr(OH)2 has antimicrobial properties against bacteria associated with peri-implantitis. 

Ort, förlag, år, upplaga, sidor
MDPI, 2021. Vol. 10, nr 2, artikel-id 150
Nyckelord [en]
antimicrobial, bacteria, peri-implantitis, strontium
Nationell ämneskategori
Odontologi
Identifikatorer
URN: urn:nbn:se:mau:diva-41149DOI: 10.3390/antibiotics10020150ISI: 000622033700001PubMedID: 33546189Scopus ID: 2-s2.0-85100658878OAI: oai:DiVA.org:mau-41149DiVA, id: diva2:1536218
Tillgänglig från: 2021-03-10 Skapad: 2021-03-10 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Ingår i avhandling
1. Antimicrobial potential of strontium against bacteria associated with peri-implantitis
Öppna denna publikation i ny flik eller fönster >>Antimicrobial potential of strontium against bacteria associated with peri-implantitis
2023 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Dental implants are nowadays a standard treatment to replace missing teeth and restore function and aesthetics. However, biological complications associated with implants, e.g., peri-implant infections, are common and jeopardize the success of treatment. The main aim of this thesis was to explore strontium (Sr) as a possible prevention strategy against peri-implant infections, since Sr has been shown to have antibacterial action and also to promote titanium (Ti) implant osseointegration. In this thesis, a systematic appraisal of the literature about the antimicrobial potential of Sr-functionalized Ti surfaces for oral applications was performed, and was followed by a series of in vitro studies assessing the antimicrobial potential of Sr against micro-organisms associated with peri-implantitis.

In Study I, the systematic appraisal of the literature resulted in an initial list of 1081 potentially relevant publications, where from nine publications from in vitro studies met the inclusion criteria. Most of the included studies showed that Sr-functionalized Ti exerted a limited immediate (i.e., 24 h) antimicrobial effect, likely due to a low Sr ion release; a relevant antimicrobial effect and biofilm inhibition potential against Streptococcus aureus was observed at both early and late timepoints, with an adequate Sr ion release.

Study II assessed, in vitro, five different concentrations of soluble Sr(OH)2 (100, 10, 1, 0.1, and 0.01 mM) against 6 different mono-species bacteria (Streptococcusmitis, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Escherichia coli, and Fusobacterium nucleatum) in terms of cell growth, minimal inhibitory concentration (MIC), and biofilm viability. In the agar diffusion test, zones of inhibition were only observed for 0.01, 0.1, and 1 mM of Sr(OH)2 against P. gingivalis. Growth inhibition in planktonic cultures was achieved at 10 mM for all species tested. In the biofilm viability assay, 10 and 100 mM Sr(OH)2 showed potent bactericidal effect against S. mitis, S. epidermidis, A. actinomycetemcomitans, E. coli, and P. gingivalis. It was thus concluded that Sr(OH)2 has antimicrobial properties against bacteria associated with peri-implantitis.

Study III assessed the in vitro early- and late bacteriostatic and bactericidal effect of Sr-functionalized wafers on bacteria associated with peri-implantitis (E. coli,S. aureus, Streptococcus oralis, Actinomyces naeslundii, Parvimonas micra, P.gingivalis and F. nucleatum) as mono-species after 2 and 24 hours, and as multispecies at day 1, 3, and 6. Sr-functionalized wafers, compared to Ti controls, were associated with statistically significant less viable cells in both mono- and multispecies tests. Number of colony forming units (CFUs) within the biofilm were significantly higher in Ti wafers, compared to Sr-functionalized wafers, for S. aureus at all time-points of evaluation and for E. coli at day 1. Gingipain activity was higher in Ti wafers compared to Sr-functionalized ones, and the qPCR showed that P. gingivalis comprised 15% of the total biofilm on Ti wafers at day 6, while it remained below detection levels at Sr-coated wafers.

In Study IV, the impact of Ti surface roughness (turned vs moderately rough) on the antimicrobial effect of Sr on bacterial associated with peri-implantitis (S. oralis, P. micra, A. naeslundii, F. nucleatum, P. gingivalis, S. aureus, and E. coli), grown in different multispecies consortia, was assessed in vitro. Bacterial viability and biofilm formation, and well as, proteolytic activity of P. gingivalis were assessed at day 1, 3, and 6. Sr-functionalized surfaces were associated with statistically significant reduction in number of viable cells compared to nonfunctionalized surface at all times of investigation for all multispecies tested. Higher proteolytic activity of P. gingivalis was found at non-functionalized Ti disks compared to Sr-functionalized ones. Sr-functionalised surfaces were associated with notable growth inhibition of both E. coli and S. aureus, while P. gingivalis remined undetected at all time points of evaluation on all disks. The turned surface had a slightly higher release of Sr ion compared with the moderately rough surface in the first 24 hours, while both surfaces showed a sustained release for up to 15 days.

Overall, the data generated with this series of projects indicate Sr surfaces exerts an antimicrobial potential on bacteria associated with peri-implantitis and it is worthwhile to further explore the potential of Sr-functionalized Ti in the prevention of peri-implant infections.

Ort, förlag, år, upplaga, sidor
Malmö: Malmö University Press, 2023. s. 76
Serie
Malmö University Odontological Dissertations, ISSN 1650-6065
Nationell ämneskategori
Odontologi
Identifikatorer
urn:nbn:se:mau:diva-58794 (URN)10.24834/isbn.9789178773718 (DOI)978-91-7877-370-1 (ISBN)978-91-7877-371-8 (ISBN)
Disputation
2023-04-27, Assembly hall, Faculty of Odontology, Malmö University, Smedjegatan 16, 13:00 (Engelska)
Opponent
Handledare
Anmärkning

Paper III and IV in dissertation as manuscript

Tillgänglig från: 2023-03-24 Skapad: 2023-03-24 Senast uppdaterad: 2024-02-29Bibliografiskt granskad

Open Access i DiVA

fulltext(3513 kB)116 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 3513 kBChecksumma SHA-512
3da29cfc72a3c6b0495afb6ea4a9c53e57ac9f53d78138e6b696bd8ef393dc61407753505b0d81c7f7af2f00ea6dcb2227691ce764505c91317879046108f21f
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Alshammari, HatemNeilands, JessicaSvensäter, GunnelStavropoulos, Andreas

Sök vidare i DiVA

Av författaren/redaktören
Alshammari, HatemNeilands, JessicaSvensäter, GunnelStavropoulos, Andreas
Av organisationen
Odontologiska fakulteten (OD)Biofilms Research Center for Biointerfaces
I samma tidskrift
Antibiotics
Odontologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 116 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 265 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf