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A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
ISIS Facility, STFC, Rutherford Appleton Laboratory, Chilton, Didcot, Oxon OX11 0QX, UK.
Institut Laue Langevin, 71 avenue des Martyrs, Grenoble 38000, France.
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2021 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 584, p. 660-668, article id S0021-9797(20)31464-8Article in journal (Refereed) Published
Abstract [en]

HYPOTHESIS: Salivary pellicles i.e., thin films formed upon selective adsorption of saliva, protect oral surfaces against chemical and mechanical insults. Pellicles are also excellent aqueous lubricants. It is generally accepted that reconstituted pellicles have a two-layer structure, where the outer layer is mainly composed of MUC5B mucins. We hypothesized that by comparing the effect of ionic strength on reconstituted pellicles and MUC5B films we could gain further insight into the pellicle structure.

EXPERIMENTS: Salivary pellicles and MUC5B films reconstituted on solid surfaces were investigated at different ionic strengths by Force Spectroscopy, Quartz Crystal Microbalance with Dissipation, Null Ellipsometry and Neutron Reflectometry.

FINDINGS: Our results support the two-layer structure for reconstituted salivary pellicles. The outer layer swelled when ionic strength decreased, indicating a weak polyelectrolyte behavior. While initially the MUC5B films exhibited a similar tendency, this was followed by a drastic collapse indicating an interaction between exposed hydrophobic domains. This suggests that mucins in the pellicle outer layer form complexes with other salivary components that prevent this interaction. Lowering ionic strength below physiological values also led to a partial removal of the pellicle inner layer. Overall, our results highlight the importance that the interactions of mucins with other pellicle components play on their structure.

Place, publisher, year, edition, pages
Elsevier, 2021. Vol. 584, p. 660-668, article id S0021-9797(20)31464-8
Keywords [en]
Ionic strength, MUC5B, Mucin, Salivary pellicle, Steric forces
National Category
Dentistry
Identifiers
URN: urn:nbn:se:mau:diva-37667DOI: 10.1016/j.jcis.2020.10.124ISI: 000600220000006PubMedID: 33198975Scopus ID: 2-s2.0-85096107333OAI: oai:DiVA.org:mau-37667DiVA, id: diva2:1511791
Available from: 2020-12-21 Created: 2020-12-21 Last updated: 2023-10-30Bibliographically approved
In thesis
1. On the structure and mechanical properties of in vitro salivary pellicles
Open this publication in new window or tab >>On the structure and mechanical properties of in vitro salivary pellicles
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Salivary pellicles display exceptional hydration and lubrication performance. At present, there are still gaps in the understanding of how this is achieved. The aim of this thesis was therefore to increase our knowledge on the mechanisms underlying these properties and deepen the understanding of how they are related to the composition and structure of pellicles, with a focus on those formed under in vitro conditions. This has applications ranging from the development of artificial saliva and lubricating coatings for biomedical applications to methodological approaches for initial testing of oral healthcare products. For this, we also focused on developing suitable methodological approaches for these studies, centering on atomic force microscopy, quartz crystal microbalance with dissipation monitoring, ellipsometry and neutron reflectometry techniques, to investigate in vitro and model salivary pellicles.

First, we confirmed a two-layer structure for in vitro salivary pellicles and showed that the outer layer is mainly composed by the oral mucin MUC5B, but that it also contains other salivary components that enhance swelling and hydration. In the presence of bulk saliva, the outer layer also contains a reversibly and loosely bound fraction. This fraction increases the adhesiveness of the pellicle but unexpectedly has no significant effect on its lubrication performance. We also investigated the effect of mechanical confinement on model salivary pellicles by means of Neutron Reflectometry, revealing that at a pressure of 1 bar they are already completely compressed and dehydrated. Finally, with the aim to advance towards better oral healthcare products, we investigated the effect of nonionic and amphoteric surfactants on salivary pellicles, showing that they have a gentler effect on pellicle structure than the commonly employed anionic surfactants.

Place, publisher, year, edition, pages
Malmö universitet, 2021. p. 59
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 2022:1
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:mau:diva-49108 (URN)10.24834/isbn.9789178772414 (DOI)978-91-7877-240-7 (ISBN)978-91-7877-241-4 (ISBN)
Public defence
2022-01-21, Live stream and on location at HS assembly hall, Malmö, 10:00 (English)
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Supervisors
Note

Paper III in dissertation as manuscript.

Available from: 2022-01-10 Created: 2022-01-06 Last updated: 2024-01-09Bibliographically approved

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Boyd, HannahGonzalez-Martinez, Juan FRobertsson, CarolinaWickström, ClaesArnebrant, ThomasSotres, Javier

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Boyd, HannahGonzalez-Martinez, Juan FRobertsson, CarolinaWickström, ClaesArnebrant, ThomasSotres, Javier
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Department of Biomedical Science (BMV)Biofilms Research Center for BiointerfacesFaculty of Odontology (OD)
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Journal of Colloid and Interface Science
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