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Sequestering of damage-associated molecular-patterns (DAMPs): a possible mechanism affecting the immune stimulating properties of aluminium adjuvants
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0003-0769-9988
2017 (English)In: Immunologic research, ISSN 0257-277X, E-ISSN 1559-0755, Vol. 65, no 6, p. 1164-1175Article in journal (Refereed)
Abstract [en]

Aluminium based adjuvants (ABAs) have been used in human and veterinary vaccines for decades and for a long time the adjuvant properties were believed to be mediated by an antigen depot at the injection site, prolonging antigen exposure to the immune system. The depot hypothesis is today more or less abandoned, and instead replaced by the assumption that ABAs induce an inflammation at the injection site. Induction of an inflammatory response is consistent with immune activation initiated by recognition of molecular patterns associated with danger or damage (DAMPs), and the latter are derived from endogenous molecules that normally reside intracellularly. When extracellularly expressed, because of damage, stress or cell death, a sterile inflammation is induced. In this paper, we report the induction of DAMP release by viable cells after phagocytosis of aluminium based adjuvants. Two of the most commonly used ABAs in pharmaceutical vaccine formulations, aluminium oxyhydroxide and aluminium hydroxyphosphate, induced a vigorous extracellular expression of the two DAMP molecules calreticulin and HMGB1. Concomitantly, extracellular adjuvant particles adsorbed the DAMP molecules released by the cells whereas IL-1, a previously reported inflammatory mediator induced by ABAs, was not absorbed by the adjuvants. Induction of extracellular expression of the two DAMP molecules was more prominent using aluminium hydroxyphosphate compared to aluminium oxyhydroxide, whereas the extracellular adsorption of the DAMP molecules was more pronounced with the latter. Furthermore, is hypothesised how induction of DAMP expression by ABAs and their concomitant adsorption by extra cellular adjuvants may affect the inflammatory properties of ABAs.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 65, no 6, p. 1164-1175
Keywords [en]
Alarmins, Aluminium based adjuvant, DAMP, lumogallion, sterile inflammation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:mau:diva-4667DOI: 10.1007/s12026-017-8972-5ISI: 000416918100010PubMedID: 29181774Scopus ID: 2-s2.0-85035149331Local ID: 24183OAI: oai:DiVA.org:mau-4667DiVA, id: diva2:1401501
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-02-05Bibliographically approved

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Sandberg, ToveEriksson, Håkan

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