Mesoporous silica particles (MSPs) have great potential to be used as drug carriers for inhalable treatments based on their physiochemical composition. MSPs are biocompatible microparticles that break down into nanoparticles when solved in biological fluids. The small size raises concerns about translocation from lung tissue into the bloodstream. The interaction of MSPs with erythrocytes is a critical point to be addressed, and the hemolytic assay is a classical test used to evaluate particle toxicity. This project used naked MSPs and MSPs loaded with an antibiotic and degraded MSPs to determine their influence on hemolytic activity. Scanning electron microscopy (SEM) was performed on erythrocytes to observe if the interaction caused changes to the cell morphology. The percentage of induced hemolysis was calculated and evaluated according to standard practice. The results showed that only the highest concentration of naked and loaded MSPs, at 25 mg/L, caused enough hemolysis to be considered slightly hemolytic but no replicate induced hemolysis above 5%. Hemolysis was not affected by using naked MSPs compared to loaded MSPs. Degraded MSPs were identified as significantly more compatible than intact MSPs and didn’t induce >2% hemolysis. There were no noticeable cell morphology changes observed through the SEM. The experiment concluded that intact MSP is not considered hemolytic under static conditions at 12.5 mg/L or lower. However, hemolytic assays using moving and dynamic flow models should be performed before a complete assessment of MSPs hemolytic activity can be made. Future studies on interactions between MSPs and various human cells and tissues will determine the medical applicability of MSPs as drug carriers for inhalable therapy.