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Dixon, J., Tubert-Jeannin, S., Davies, J. R., van Harten, M., Roger-Leroi, V., Vital, S., . . . Field, J. (2024). O-Health-Edu : A viewpoint into the current state of oral health professional education in Europe: Part 2: Curriculum structure, facilities, staffing and quality assurance. European journal of dental education
Open this publication in new window or tab >>O-Health-Edu : A viewpoint into the current state of oral health professional education in Europe: Part 2: Curriculum structure, facilities, staffing and quality assurance
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2024 (English)In: European journal of dental education, ISSN 1396-5883, E-ISSN 1600-0579Article in journal (Refereed) Epub ahead of print
Abstract [en]

INTRODUCTION: Oral health professional (OHP) education is likely to vary across Europe in accordance with an EU directive that is open to broad interpretation. It is not clear how OHP curricula are structured or delivered across Europe. The objectives of Part 2 of this paper series are: (i) to provide an overview of common practices in curriculum structure, the availability of facilities, staffing (faculty) and quality assurance processes and (ii) to consider how the existing programme structures align to stakeholder guidance documents.

METHODS: A total of 27 questions from a 91-item questionnaire were used for this manuscript. The questionnaire was developed following the Delphi method to establish consensus from a group of experts. Members of the research team and colleagues from other countries in Europe completed a multi-step piloting process. An online data hub was created to allow the respondents to be data controllers and respond to the questionnaire. ADEE member schools (n = 144) were invited to provide data.

RESULTS: Totally, 71 institutions from 25 European countries provided data between June 2021 and April 2023, which represents a response rate of 49.3% of ADEE members. Data on curriculum approaches, teaching methods, integration of topics of interest, clinical education, staff-student ratios, access to facilities and new technologies, teaching staff (faculty) and quality assurance processes are presented for Primary Dental Degree Programmes.

CONCLUSION: To the best of our knowledge, this series of papers are the first attempts to provide a comprehensive overview of OHP education in Europe. Results showed that the majority of European dental programmes are engaged in providing innovative and scientifically grounded education in order to develop quality future OHPs. Nevertheless, significant variability in the delivery of clinical education across the European OHP schools was notable in this dataset. A comprehensive view of the state of OHP education in Europe is not yet available but the O-Health-Edu data hub provides a means for all education providers in Europe to contribute data to reach this goal. It is anticipated that the data hub will be updated and built upon over time to continually establish a clearer picture of the state of OHP education in Europe.

Place, publisher, year, edition, pages
John Wiley & Sons, 2024
Keywords
Europe, dental education, dental hygienists, oral health professionals, survey
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-65694 (URN)10.1111/eje.12987 (DOI)001155062200001 ()38258340 (PubMedID)2-s2.0-85182838323 (Scopus ID)
Available from: 2024-02-02 Created: 2024-02-02 Last updated: 2024-02-27Bibliographically approved
Dixon, J., Field, J., Vital, S., van Harten, M., Roger-Leroi, V., Davies, J. R., . . . Tubert-Jeannin, S. (2024). O-HEALTH-EDU: A viewpoint into the current state of Oral Health Professional education in Europe: Part 1: Programme-level data. European journal of dental education
Open this publication in new window or tab >>O-HEALTH-EDU: A viewpoint into the current state of Oral Health Professional education in Europe: Part 1: Programme-level data
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2024 (English)In: European journal of dental education, ISSN 1396-5883, E-ISSN 1600-0579Article in journal (Refereed) Epub ahead of print
Abstract [en]

Introduction: Current legislation leaves Oral Health Professional (OHP) education open to wide interpretation and may result in significant variation in educational practice and resultant professional attributes across Europe. Data regarding the current state of OHP education across Europe is limited. The aim of Part 1 of this series is to provide programme-level data for Primary Dental Degree Programmes, Dental Hygiene and Postgraduate Education.Methods: A 91-item questionnaire was developed following the Delphi method. The questionnaire and the Articulate glossary of OHP education terms were developed concurrently to facilitate a common understanding of language. Piloting was performed in multiple stages and included institutions internal and external to the research group. The questionnaire was uploaded online and converted to a data hub, allowing dental schools to control their own data and update the data provided whenever they wish. All ADEE member schools (n = 144) were invited to provide data. Forty questions relating to school details, Primary Dental Degree Programmes, Dental Hygiene and Postgraduate Education were included in this part of the series.Results: Seventy-one institutions from 25 European countries provided data between June 2021 and April 2023, which represents a response rate of 49.3% of ADEE members. Programme-level data for Primary Dental Degree Programmes, Dental Hygiene and Postgraduate Education is presented including programme length, funding, languages and fees, student numbers and demographics, student admission and selection processes and permission to practice after graduation.Conclusion: This series of papers, as far as the authors are aware, are the first attempts to build a comprehensive picture of the current state of OHP education in Europe. A comprehensive view of the state of OHP education in Europe is not yet available but the O-Health-Edu data hub provides a means for all education providers in Europe to contribute data to reach this goal. It is anticipated that the data hub will be updated and built upon over time to continually establish a clearer picture of the state of OHP education in Europe.

Place, publisher, year, edition, pages
John Wiley & Sons, 2024
Keywords
dental education, dental hygienists, Europe, Oral Health Professionals, survey
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-65500 (URN)10.1111/eje.12989 (DOI)001137512400001 ()38186364 (PubMedID)2-s2.0-85181652237 (Scopus ID)
Available from: 2024-02-01 Created: 2024-02-01 Last updated: 2024-02-02Bibliographically approved
Davies, J. R., Field, J., Dixon, J., Manzanares-Cespedes, M.-C., Vital, S., Paganelli, C., . . . Tubert-Jeannin, S. (2023). ARTICULATE: A European glossary of terms used in oral health professional education. European journal of dental education, 27(2), 209-222
Open this publication in new window or tab >>ARTICULATE: A European glossary of terms used in oral health professional education
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2023 (English)In: European journal of dental education, ISSN 1396-5883, E-ISSN 1600-0579, Vol. 27, no 2, p. 209-222Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: The Erasmus+O-Health-EDU project aims to gain a comprehensive view of oral health professional (OHP) education in Europe, through the development of web-based surveys and online toolkits. A glossary to facilitate a common language through which academic teams could cooperate and communicate more accurately was identified as a key need within the project. The aim of ARTICULATE was thus to create a shared language, with a European focus, for terms and concepts used in the field of OHP education.

METHODS: The methodology was developed from those published for construction of other glossaries with a circular and iterative process: the creation of content and definitions by a group of experts in OHP education, the testing of "fitness for purpose" of the content, and stakeholder consultation. All creation steps were followed by refinements based on testing results and stakeholder comments. The final glossary was then launched as an online resource including a built-in mechanism for user feedback.

RESULTS: The scope and structure of the glossary were mapped out at a workshop with 12 dental education experts from 7 European countries. A total of 328 terms were identified, of which 171 were finally included in ARTICULATE. After piloting with a close group of other colleagues, the glossary was opened for external input. Thirty European Deans or Heads of Education assessed the definition of each term as "clear" or "not clear." A total of 86 definitions were described as "clear" by all individuals. Terms deemed unclear by at least one individual were revisited and changes made to 37 of the definitions. In conjunction with the launch of the glossary, a range of stakeholder organisations were informed and asked to participate in an open global consultation by providing feedback online. Since its launch in June 2021, the ARTICULATE website (https://o-health-edu.org/articulate) has had an average of 500 visits/month. To promote community ownership, forms embedded on the ARTICULATE webpage allow users to give feedback and suggest new terms. A standing taskforce will meet regularly to consider amendments and make changes to ensure that the glossary remains a relevant and up-to-date resource over time.

CONCLUSION: ARTICULATE is a unique, evolving, online glossary of terms relating to OHP education, created as a resource for all interested OHP educators. The glossary is a key output of the O-Health-Edu project, which relies on a comprehensive vision of OHP education to address the future oral health needs of the European population.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
Europe, dictionary, lexicon, oral health, professional education, terminology
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-50544 (URN)10.1111/eje.12794 (DOI)000766035900001 ()35224823 (PubMedID)2-s2.0-85125854561 (Scopus ID)
Available from: 2022-03-10 Created: 2022-03-10 Last updated: 2023-04-20Bibliographically approved
Neilands, J., Svensäter, G., Boisen, G., Robertsson, C., Wickström, C. & Davies, J. R. (2023). Formation and Analysis of Mono-species and Polymicrobial Oral Biofilms in Flow-Cell Models. In: Bacterial Pathogenesis: Methods and Protocols, (pp. 33-52). Springer
Open this publication in new window or tab >>Formation and Analysis of Mono-species and Polymicrobial Oral Biofilms in Flow-Cell Models
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2023 (English)In: Bacterial Pathogenesis: Methods and Protocols,, Springer, 2023, p. 33-52Chapter in book (Refereed)
Abstract [en]

The oral microbiota, which is known to include at least 600 different bacterial species, is found on the teethand mucosal surfaces as multi-species communities or biofilms. The oral surfaces are covered with a pellicleof proteins absorbed from saliva, and biofilm formation is initiated when primary colonizers, which expresssurface adhesins that bind to specific salivary components, attach to the oral tissues. Further developmentthen proceeds through co-aggregation of additional species. Over time, the composition of oral biofilms,which varies between different sites throughout the oral cavity, is determined by a combination ofenvironmental factors such as the properties of the underlying surface, nutrient availability and oxygenlevels, and bacterial interactions within the community. A complex equilibrium between biofilm communities and the host is responsible for the maintenance of a healthy biofilm phenotype (eubiosis). In the faceof sustained environmental perturbation, however, biofilm homeostasis can break down giving rise todysbiosis, which is associated with the development of oral diseases such as caries and periodontitis.In vitro models have an important part to play in increasing our understanding of the complex processesinvolved in biofilm development in oral health and disease, and the requirements for experimental system,microbial complexity, and analysis techniques will necessarily vary depending on the question posed. In thischapter we describe some current and well-established methods used in our laboratory for studying oralbacteria in biofilm models which can be adapted to suit the needs of individual users. 

Place, publisher, year, edition, pages
Springer, 2023
Series
Methods in Molecular Biology, E-ISSN 1940-6029 ; 2674
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:mau:diva-62874 (URN)10.1007/978-1-0716-3243-7_2 (DOI)37258958 (PubMedID)2-s2.0-85160680476 (Scopus ID)978-1-0716-3242-0 (ISBN)978-1-0716-3243-7 (ISBN)
Available from: 2023-09-29 Created: 2023-09-29 Last updated: 2023-10-06Bibliographically approved
Boisen, G., Prgomet, Z., Enggren, G., Dahl, H., Mkadmi, C. & Davies, J. R. (2023). Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria. Biofilm, 6, Article ID 100136.
Open this publication in new window or tab >>Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria
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2023 (English)In: Biofilm, E-ISSN 2590-2075, Vol. 6, article id 100136Article in journal (Refereed) Published
Abstract [en]

Probiotic bacteria show promising results in prevention of the biofilm-mediated disease caries, but the mechanisms are not fully understood. The acid tolerance response (ATR) allows biofilm bacteria to survive and metabolize at low pH resulting from microbial carbohydrate fermentation. We have studied the effect of probiotic strains: Limosilactobacillus reuteri and Lacticaseibacillus rhamnosus on ATR induction in common oral bacteria. Communities of L. reuteri ATCC PTA5289 and Streptoccus gordonii, Streptococcus oralis, Streptococcus mutans or Actinomyces naeslundii in the initial stages of biofilm formation were exposed to pH 5.5 to allow ATR induction, followed by a low pH challenge. Acid tolerance was evaluated as viable cells after staining with LIVE/ DEAD & REG;BacLightTM. The presence of L. reuteri ATCC PTA5289 caused a significant reduction in acid tolerance in all strains except S. oralis. When S. mutans was used as a model organism to study the effects of additional probiotic strains (L. reuteri SD2112, L. reuteri DSM17938 or L. rhamnosus GG) as well as L. reuteri ATCC PTA5289 supernatant on ATR development, neither the other probiotic strains nor supernatants showed any effect. The presence of L. reuteri ATCC PTA5289 during ATR induction led to down-regulation of three key genes involved in tolerance of acid stress (luxS, brpA and ldh) in Streptococci. These data suggest that live cells of probiotic L. reuteri ATCC PTA5289 can interfere with ATR development in common oral bacteria and specific strains of L. reuteri may thus have a role in caries prevention by inhibiting development of an acid-tolerant biofilm microbiota.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Probiotics, Acid tolerance, Caries, Early oral biofilms, Pioneer species
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-61921 (URN)10.1016/j.bioflm.2023.100136 (DOI)001038416000001 ()37408693 (PubMedID)2-s2.0-85163191080 (Scopus ID)
Available from: 2023-08-16 Created: 2023-08-16 Last updated: 2023-12-07Bibliographically approved
Hix Janssens, T., Shinde, S., Abouhany, R., Davies, J. R., Neilands, J., Svensäter, G. & Sellergren, B. (2023). Microcontact-Imprinted Optical Sensors for Virulence Factors of Periodontal Disease. ACS Omega, 8(17), 15259-15265
Open this publication in new window or tab >>Microcontact-Imprinted Optical Sensors for Virulence Factors of Periodontal Disease
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2023 (English)In: ACS Omega, E-ISSN 2470-1343, Vol. 8, no 17, p. 15259-15265Article in journal (Refereed) Published
Abstract [en]

Periodontitis (gum disease) is a common biofilm-mediated oral condition, with around 7% of the adult population suffering from severe disease with risk for tooth loss. Moreover, periodontitis virulence markers have been found in atherosclerotic plaque and brain tissue, suggesting a link to cardiovascular and Alzheimer’s diseases. The lack of accurate, fast, and sensitive clinical methods to identify patients at risk leads, on the one hand, to patients being undiagnosed until the onset of severe disease and, on the other hand, to overtreatment of individuals with mild disease, diverting resources from those patients most in need. The periodontitis-associated bacterium, Porphyromonas gingivalis, secrete gingipains which are highly active proteases recognized as key virulence factors during disease progression. This makes them interesting candidates as predictive biomarkers, but currently, there are no methods in clinical use for monitoring them. Quantifying the levels or proteolytic activity of gingipains in the periodontal pocket surrounding the teeth could enable early-stage disease diagnosis. Here, we report on a monitoring approach based on high-affinity microcontact imprinted polymer-based receptors for the Arg and Lys specific gingipains Rgp and Kgp and their combination with surface plasmon resonance (SPR)-based biosensor technology for quantifying gingipain levels in biofluids and patient samples. Therefore, Rgp and Kgp were immobilized on glass coverslips followed by microcontact imprinting of poly-acrylamide based films anchored to gold sensor chips. The monomers selected were N-isopropyl acrylamide (NIPAM), N-hydroxyethyl acrylamide (HEAA) and N-methacryloyl-4-aminobenzamidine hydrochloride (BAM), with N,N′-methylene bis(acrylamide) (BIS) as the crosslinker. This resulted in imprinted surfaces exhibiting selectivity towards their templates high affinity and selectivity for the templated proteins with dissociation constants (Kd) of 159 and 299 nM for the Rgp- and Kgp-imprinted, surfaces respectively. The former surface displayed even higher affinity (Kd = 71 nM) when tested in dilute cell culture supernatants. Calculated limits of detection for the sensors were 110 and 90 nM corresponding to levels below clinically relevant concentrations.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
National Category
Dentistry Medical Biotechnology
Identifiers
urn:nbn:se:mau:diva-59511 (URN)10.1021/acsomega.3c00389 (DOI)000978106200001 ()37151489 (PubMedID)2-s2.0-85154067619 (Scopus ID)
Available from: 2023-05-15 Created: 2023-05-15 Last updated: 2023-08-15Bibliographically approved
Field, J., Dixon, J., Davies, J. R., Quinn, B., Murphy, D., Vital, S., . . . Tubert-Jeannin, S. (2023). O-Health-Edu: A vision for oral health professional education in Europe. [Letter to the editor]. European journal of dental education, 27(2), 382-387
Open this publication in new window or tab >>O-Health-Edu: A vision for oral health professional education in Europe.
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2023 (English)In: European journal of dental education, ISSN 1396-5883, E-ISSN 1600-0579, Vol. 27, no 2, p. 382-387Article in journal, Letter (Other academic) Published
Abstract [en]

This consensus paper reports on the process of developing a renewed vision for Oral Health Professional (OHP) education across Europe, and forms part of a larger EU-funded collaborative Erasmus+ project, "O-Health-Edu." The vision aligns with the World Health Organisation milestones (2016) and resolutions (2021), and EU4Health programme (2020) objectives - and projects 20 years into the future, to 2040. This longitudinal vision takes a multi-stakeholder perspective to deliver OHP education that acts in the best interests of both students and patients, and sits within the context of a wider strategy for general health. Included, it is an infographic to help communicate the vision to various stakeholders of OHP education.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
Europe, World Health Organisation, consensus, education, oral health, students
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-52202 (URN)10.1111/eje.12819 (DOI)000806201200001 ()35661367 (PubMedID)2-s2.0-85131266360 (Scopus ID)
Available from: 2022-06-08 Created: 2022-06-08 Last updated: 2023-04-20Bibliographically approved
Robertsson, C., Svensäter, G., Davies, J. R., Bay Nord, A., Malmodin, D. & Wickström, C. (2023). Synergistic metabolism of salivary MUC5B in oral commensal bacteria during early biofilm formation. Microbiology Spectrum, 11(6)
Open this publication in new window or tab >>Synergistic metabolism of salivary MUC5B in oral commensal bacteria during early biofilm formation
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2023 (English)In: Microbiology Spectrum, E-ISSN 2165-0497, Vol. 11, no 6Article in journal (Refereed) Published
Abstract [en]

Bacterial metabolism in oral biofilms is comprised of complex networks of nutritional chains and biochemical regulations. These processes involve both intraspecies and interspecies networks as well as interactions with components from host saliva, gingival crevicular fluid, and dietary intake. In a previous paper, a large salivary glycoprotein, mucin MUC5B, was suggested to promote a dental health-related phenotype in the oral type strain of Streptococcus gordonii DL1, by regulating bacterial adhesion and protein expression. In this study, nuclear magnetic resonance-based metabolomics was used to examine the effects on the metabolic output of monospecies compared to dual species early biofilms of two clinical strains of oral commensal bacteria, S. gordonii and Actinomyces naeslundii, in the presence of MUC5B. The presence of S. gordonii increased colonization of A. naeslundii on salivary MUC5B, and both commensals were able to utilize MUC5B as a sole nutrient source during early biofilm formation. The metabolomes suggested that the bacteria were able to release mucin carbohydrates from oligosaccharide side chains as well as amino acids from the protein core. Synergistic effects were also seen in the dual species biofilm metabolome compared to the monospecies, indicating that A. naeslundii and S. gordonii cooperated in the degradation of salivary MUC5B. A better understanding of bacterial interactions and salivary-mediated regulation of early dental biofilm activity is meaningful for understanding oral biofilm physiology and may contribute to the development of future prevention strategies for biofilm-induced oral disease.

IMPORTANCE: The study of bacterial interactions and salivary-mediated regulation of early dental biofilm activity is of interest for understanding oral microbial adaptation to environmental cues and biofilm maturation. Findings in oral commensals can prove useful from the perspectives of both oral and systemic health of the host, as well as the understanding of general microbial biofilm physiology. The knowledge may provide a basis for the development of prognostic biomarkers, or development of new treatment strategies, related to oral health and disease and possibly also to other biofilm-induced conditions. The study is also an important step toward developing the methodology for similar studies in other species and/or growth conditions.

Place, publisher, year, edition, pages
ASM International, 2023
Keywords
MUC5B, NMR, actinomyces, bacterial metabolism, biofilm physiology, dental biofilm, metabolomics, oral microbiology, saliva, streptococci
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-63213 (URN)10.1128/spectrum.02704-23 (DOI)001085549500001 ()37855449 (PubMedID)2-s2.0-85180007534 (Scopus ID)
Available from: 2023-10-23 Created: 2023-10-23 Last updated: 2024-01-10Bibliographically approved
Riaz, A., Gidvall, S., Prgomet, Z., Hernandez, A. R., Ruzgas, T., Nilsson, E. J., . . . Valetti, S. (2023). Three-Dimensional Oral Mucosal Equivalents as Models for Transmucosal Drug Permeation Studies. Pharmaceutics, 15(5), 1513-1513
Open this publication in new window or tab >>Three-Dimensional Oral Mucosal Equivalents as Models for Transmucosal Drug Permeation Studies
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2023 (English)In: Pharmaceutics, ISSN 1999-4923, E-ISSN 1999-4923, Vol. 15, no 5, p. 1513-1513Article in journal (Refereed) Published
Abstract [en]

Oral transmucosal administration, where drugs are absorbed directly through the non-keratinized, lining mucosa of the mouth, represents a solution to drug delivery with several advantages. Oral mucosal equivalents (OME) developed as 3D in vitro models are of great interest since they express the correct cell differentiation and tissue architecture, simulating the in vivo conditions better than monolayer cultures or animal tissues. The aim of this work was to develop OME to be used as a membrane for drug permeation studies. We developed both full-thickness (i.e., connective plus epithelial tissue) and split-thickness (i.e., only epithelial tissue) OME using non-tumor-derived human keratinocytes OKF6 TERT-2 obtained from the floor of the mouth. All the OME developed here presented similar transepithelial electrical resistance (TEER) values, comparable to the commercial EpiOral™. Using eletriptan hydrobromide as a model drug, we found that the full-thickness OME had similar drug flux to EpiOral™ (28.8 vs. 29.6 µg/cm2/h), suggesting that the model had the same permeation barrier properties. Furthermore, full-thickness OME showed an increase in ceramide content together with a decrease in phospholipids in comparison to the monolayer culture, indicating that lipid differentiation occurred due to the tissue-engineering protocols. The split-thickness mucosal model resulted in 4–5 cell layers with basal cells still undergoing mitosis. The optimum period at the air–liquid interface for this model was twenty-one days; after longer times, signs of apoptosis appeared. Following the 3R principles, we found that the addition of Ca2+, retinoic acid, linoleic acid, epidermal growth factor and bovine pituitary extract was important but not sufficient to fully replace the fetal bovine serum. Finally, the OME models presented here offer a longer shelf-life than the pre-existing models, which paves the way for the further investigation of broader pharmaceutical applications (i.e., long-term drug exposure, effect on the keratinocytes’ differentiation and inflammatory conditions, etc.).

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
oral transmucosal delivery, oral mucosal equivalents, drug permeation, 3R principles, 3D in vitro models
National Category
Pharmaceutical Sciences
Identifiers
urn:nbn:se:mau:diva-61046 (URN)10.3390/pharmaceutics15051513 (DOI)000997495400001 ()37242755 (PubMedID)2-s2.0-85160448981 (Scopus ID)
Funder
The Crafoord Foundation, 20210937Knowledge Foundation, 20190010
Available from: 2023-06-19 Created: 2023-06-19 Last updated: 2023-08-15Bibliographically approved
Aherne, O., Ortiz, R., Fazli, M. M. & Davies, J. R. (2022). Effects of stabilized hypochlorous acid on oral biofilm bacteria. BMC Oral Health, 22(1), Article ID 415.
Open this publication in new window or tab >>Effects of stabilized hypochlorous acid on oral biofilm bacteria
2022 (English)In: BMC Oral Health, ISSN 1472-6831, E-ISSN 1472-6831, Vol. 22, no 1, article id 415Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Caries and periodontitis are amongst the most prevalent diseases worldwide, leading to pain and loss of oral function for those affected. Prevention relies heavily on mechanical removal of dental plaque biofilms but for populations where this is not achievable, alternative plaque control methods are required. With concerns over undesirable side-effects and potential bacterial resistance due to the use of chlorhexidine gluconate (CHX), new antimicrobial substances for oral use are greatly needed. Here we have investigated the antimicrobial effect of hypochlorous acid (HOCl), stabilized with acetic acid (HAc), on oral biofilms and compared it to that of CHX. Possible adverse effects of stabilized HOCl on hydroxyapatite surfaces were also examined.

METHODS: Single- and mixed-species biofilms of six common oral bacteria (Streptococcus mutans, Streptococcus gordonii, Actinomyces odontolyticus, Veillonella parvula, Parvimonas micra and Porphyromonas gingivalis) within a flow-cell model were exposed to HOCl stabilized with 0.14% or 2% HAc, pH 4.6, as well as HOCl or HAc alone. Biofilm viability was assessed in situ using confocal laser scanning microscopy following LIVE/DEAD® BacLight™ staining. In-situ quartz crystal microbalance with dissipation (QCM-D) was used to study erosion of hydroxyapatite (HA) surfaces by stabilized HOCl.

RESULTS: Low concentrations of HOCl (5 ppm), stabilized with 0.14% or 2% HAc, significantly reduced viability in multi-species biofilms representing supra- and sub-gingival oral communities, after 5 min, without causing erosion of HA surfaces. No equivalent antimicrobial effect was seen for CHX. Gram-positive and Gram-negative bacteria showed no significant differential suceptibility to stabilized HOCl.

CONCLUSIONS: At low concentrations and with exposure times which could be achieved through oral rinsing, HOCl stabilized with HAc had a robust antimicrobial activity on oral biofilms, without causing erosion of HA surfaces or affecting viability of oral keratinocytes. This substance thus appears to offer potential for prevention and/or treatment of oral biofilm-mediated diseases.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2022
Keywords
Biofilm control, Caries, Oral disease, Oral infection, Periodontitis
National Category
Dentistry
Identifiers
urn:nbn:se:mau:diva-55178 (URN)10.1186/s12903-022-02453-2 (DOI)000855772700004 ()36127658 (PubMedID)2-s2.0-85138179900 (Scopus ID)
Available from: 2022-10-17 Created: 2022-10-17 Last updated: 2024-02-05Bibliographically approved
Projects
Lipoprotein modifications by periodontal pathogens; Malmö University, Biofilms Research Center for BiointerfacesRegulation of Surface Protein - Presentation on Streptococcus gordonii
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-5888-664X

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