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Psotta, C., Cirovic, S., Gudmundsson, P., Falk, M., Mandal, T., Reichhart, T., . . . Shleev, S. (2023). Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica. Bioelectrochemistry, 152, Article ID 108441.
Open this publication in new window or tab >>Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica
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2023 (English)In: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 152, article id 108441Article in journal (Refereed) Published
Abstract [en]

Managing blood glucose can affect important clinical outcomes during the intraoperative phase of surgery. However, currently available instruments for glucose monitoring during surgery are few and not optimized for the specific application. Here we report an attempt to exploit an enzymatic sensor in a vein replica that could continuously monitor glucose level in an authentic human bloodstream. First, detailed investigations of the superficial venous systems of volunteers were carried out using ocular and palpating examinations, as well as advanced ultrasound measurements. Second, a tubular glucose-sensitive biosensor mimicking a venous system was designed and tested. Almost ideal linear dependence of current output on glucose concentration in phosphate buffer saline was obtained in the range 2.2-22.0 mM, whereas the dependence in human plasma was less linear. Finally, the developed biosensor was investigated in whole blood under homeostatic conditions. A specific correlation was found between the current output and glucose concentration at the initial stage of the biodevice operation. However, with time, blood coagulation during measurements negatively affected the performance of the biodevice. When the experimental results were remodeled to predict the response without the influence of blood coagulation, the sensor output closely followed the blood glucose level.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Continuous glucose sensing, Enzymatic sensor, Vein replica, Human physiological fluids, Surgery
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:mau:diva-61052 (URN)10.1016/j.bioelechem.2023.108441 (DOI)000984583000001 ()37087795 (PubMedID)2-s2.0-85153044643 (Scopus ID)
Available from: 2023-06-20 Created: 2023-06-20 Last updated: 2023-10-10Bibliographically approved
Falk, M., Psotta, C., Cirovic, S., Ohlsson, L. & Shleev, S. (2023). Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study. Biosensors, 13(7), Article ID 717.
Open this publication in new window or tab >>Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study
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2023 (English)In: Biosensors, ISSN 2079-6374, Vol. 13, no 7, article id 717Article in journal (Refereed) Published
Abstract [en]

An electronic tongue is a powerful analytical instrument based on an array of non-selective chemical sensors with a partial specificity for data gathering and advanced pattern recognition methods for data analysis. Connecting electronic tongues with electrochemical techniques for data collection has led to various applications, mostly within sensing for food quality and environmental monitoring, but also in biomedical research for the analyses of different bioanalytes in human physiological fluids. In this paper, an electronic tongue consisting of six electrodes (viz., gold, platinum, palladium, titanium, iridium, and glassy carbon) was designed and tested in authentic (undiluted, unpretreated) human saliva samples from eight volunteers, collected before and during the COVID-19 pandemic. Investigations of 11 samples using differential pulse voltammetry and a principal component analysis allowed us to distinguish between SARS-CoV-2-free and infected authentic human saliva. This work, as a proof-of-principle demonstration, provides a new perspective for the use of electronic tongues in the field of enzyme-free electrochemical biosensing, highlighting their potential for future applications in non-invasive biomedical analyses.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
electronic tongue, differential pulse voltammetry, principial component analysis, authentic human saliva, SARS-CoV-2
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-61908 (URN)10.3390/bios13070717 (DOI)001038044400001 ()37504115 (PubMedID)2-s2.0-85165896609 (Scopus ID)
Available from: 2023-08-16 Created: 2023-08-16 Last updated: 2023-10-10Bibliographically approved
Ramji, R., Rämgård, M., Carlson, E., Shleev, S., Awad, E., Cirovic, S. & Kottorp, A. (2023). Health and quality of life among women after participation in a CBPR-informed physical activity intervention: with a pandemic perspective.. Scientific Reports, 13(1), Article ID 17972.
Open this publication in new window or tab >>Health and quality of life among women after participation in a CBPR-informed physical activity intervention: with a pandemic perspective.
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2023 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 13, no 1, article id 17972Article in journal (Refereed) Published
Abstract [en]

The lack of culturally and contextually oriented interventions promoting physical activity (PA) has led to increased physical inactivity among women living in disadvantaged neighbourhoods in Sweden. In this study one such intervention informed by community-based participatory research (CBPR) has been evaluated among 34 women from a disadvantaged neighbourhood before and during COVID-19. Health-related quality of life (HRQOL), behavioural and biomedical outcomes were assessed directly prior and post-intervention, followed by evaluations at 6-months and 18-months follow-up during COVID-19. The results revealed that HRQOL, particularly psychological, social, and environmental health significantly increased post-intervention compared to prior to intervention but reversed back at 6-months follow-up. Perceived health satisfaction and environmental health increased at 18-months follow-up during COVID-19. Participation in PA improved post-intervention and at 6-months follow-up. Everyday activities and fruit and vegetable intake continued to increase through all timepoints. Systolic blood pressure significantly decreased post-intervention and 6-months follow-up; blood flow rate increased significantly at all timepoints. Overall, the findings underscores the potential effectiveness of CBPR approaches in promoting and sustaining healthy lifestyles, even during acute situations such as the COVID-19. It may even serve as a future model for promoting health and addressing health disparities in similar groups.

Place, publisher, year, edition, pages
Springer Nature, 2023
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:mau:diva-63606 (URN)10.1038/s41598-023-45239-4 (DOI)001087596300084 ()37863947 (PubMedID)2-s2.0-85174618667 (Scopus ID)
Available from: 2023-11-10 Created: 2023-11-10 Last updated: 2023-12-05Bibliographically approved
Aleksejeva, O., Nilsson, N., Genevskiy, V., Thulin, K. & Shleev, S. (2022). Dual-feature photobioanodes based on nanoimprint lithography for photoelectric biosupercapacitors. Journal of Power Sources, 517, Article ID 230677.
Open this publication in new window or tab >>Dual-feature photobioanodes based on nanoimprint lithography for photoelectric biosupercapacitors
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2022 (English)In: Journal of Power Sources, ISSN 0378-7753, E-ISSN 1873-2755, Vol. 517, article id 230677Article in journal (Refereed) Published
Abstract [en]

Direct transformation of solar energy into electrical energy by means of biological photosynthesis is considered as an attractive option for sustainable electrical energy production. Thylakoid membranes, the site of photosynthesis, are regarded as a promising biological material for the development of photoelectric biodevices, which produce electrical power consuming only light energy as oxygen evolves at photobioanode upon irradiation and biocathode converts it back to water. Therefore, in this work dual-feature photobioanode based on nanoimprinted gold substrates modified with thylakoids in combination with a capacitive part made of a planar gold substrate coated with a conductive polymer was designed and evaluated, providing open-circuit potential of -0.21 V vs. Ag vertical bar AgCl vertical bar KClsat and a capacitance of ca. 60 F m(-2) both at ambient light and artificial illumination of 400 W m(-2). Combination of thylakoid based dual-feature photobioanode with bilirubin oxidase modified transparent and capacitive indium tin oxide biocathode resulted in a photoelectric biosupercapacitor with remarkable characteristics at ambient light, viz. an open-circuit voltage as high as 0.74 V, which was stable upon charge-discharge cycles during ca. 2 h.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Biosupercapacitor, Photosynthetic electricity, Thylakoid membranes, Nanoimprint lithography, Ambient light
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:mau:diva-47228 (URN)10.1016/j.jpowsour.2021.230677 (DOI)000720574900003 ()2-s2.0-85118498184 (Scopus ID)
Available from: 2021-12-06 Created: 2021-12-06 Last updated: 2024-02-05Bibliographically approved
Ramonas, E., Butkevicius, M., Shleev, S., Dagys, M. & Ratautas, D. (2022). Mechanistic characterization of an oxygen reduction reaction-driven, fully enzymatic and self-calibrating pH biosensor based on wired bilirubin oxidase. Sensors and actuators. B, Chemical, 367, Article ID 132054.
Open this publication in new window or tab >>Mechanistic characterization of an oxygen reduction reaction-driven, fully enzymatic and self-calibrating pH biosensor based on wired bilirubin oxidase
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2022 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 367, article id 132054Article in journal (Refereed) Published
Abstract [en]

The oxygen reduction reaction (ORR) is important due to high electrochemical potential. Multicopper oxidases (e.g., bilirubin oxidase (BOx)) belong to a class of enzymes that catalyze ORR with low overpotential, and have attracted significant attention in designing bioelectronic devices, viz., biosensors and enzymatic cathodes of biofuel cells. In this work, we present a new concept - an enzyme driven pH electrode system composed of two electrodes: a pH-sensitive BOx-based working electrode and pH-insensitive, a BOx-based reference electrode. ORR-driven pH monitoring was conducted by measuring the potential difference between the two electrodes. Moreover, the electrode system was self-supported and self-calibrating, and no additional electrodes (Pt counter or Ag/AgCl reference) were needed for measurements. The BOx-based pH sensor was tested in continuous and real-time pH monitoring with high accuracy. Also, we present an in-depth mechanistic study explaining the electrode potential-pH dependence, which brings new insights into the operational principle of bilirubin oxidase at different pH.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Bilirubin oxidase, Enzyme electrode, pH electrode, Oxygen reduction reaction, Reference electrode, Self-referencing electrode
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-54054 (URN)10.1016/j.snb.2022.132054 (DOI)000807773900004 ()2-s2.0-85133977564 (Scopus ID)
Available from: 2022-08-02 Created: 2022-08-02 Last updated: 2024-02-05Bibliographically approved
Aleksejeva, O., Nilsson, N., Genevskiy, V., Thulin, K. & Shleev, S. (2022). Photobioanodes Based on Nanoimprinted Electrodes and Immobilized Chloroplasts. ChemElectroChem, 9(2), 37-42
Open this publication in new window or tab >>Photobioanodes Based on Nanoimprinted Electrodes and Immobilized Chloroplasts
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2022 (English)In: ChemElectroChem, E-ISSN 2196-0216, Vol. 9, no 2, p. 37-42Article in journal (Refereed) Published
Abstract [en]

As the global energy demand continues to increase, the interest in photosynthetic energy conversion is growing accordingly. Chloroplasts, photosynthetic organelles present in plants and algae, are attractive candidates for construction of bio solar cells; however, they have been less studied because of their complex membrane system, which restricts electrochemical communication with an electrode surface. Nevertheless, in this work photobioanodes based on planar and nanoimprinted gold substrates modified with chloroplasts were designed and evaluated. Apparently, nanoimprint lithography contributed to higher photocurrent densities, not only owing to the enlarged real surface area, but also due to boosting electrochemical communication between the photosynthetic organelles and the electrode. Combining chloroplast-modified nanoimprinted gold electrodes with a capacitive part made of a planar gold substrate, coated with a conductive polymer, resulted in a dual-feature photobioanode providing a lower open-circuit potential, i. e., -0.11 V vs. Ag|AgCl|KClsat, and an enhanced capacitance of ca. 37 F m(-2) upon illumination of 400 W m(-2).

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
Keywords
Biocatalysis, Chloroplasts, Nanoimprint lithography, Photobioanode, Photosynthesis
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-47229 (URN)10.1002/celc.202101219 (DOI)000720405900001 ()2-s2.0-85119359298 (Scopus ID)
Available from: 2021-12-06 Created: 2021-12-06 Last updated: 2024-02-05Bibliographically approved
Aleksejeva, O., Sokolov, A. V., Marquez, I., Gustafsson, A., Bushnev, S., Eriksson, H., . . . Shleev, S. (2021). Autotolerant ceruloplasmin based biocathodes for implanted biological power sources. Bioelectrochemistry, 140
Open this publication in new window or tab >>Autotolerant ceruloplasmin based biocathodes for implanted biological power sources
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2021 (English)In: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 140Article in journal (Refereed) Published
Abstract [en]

High-performance autotolerant bioelectrodes should be ideally suited to design implantable bioelectronic devices. Because of its high redox potential and ability to reduce oxygen directly to water, human ceruloplasmin, HCp, the only blue multicopper oxidase present in human plasma, appears to be the ultimate biocatalyst for oxygen biosensors and also biocathodes in biological power sources. In comparison to fungal and plant blue multicopper oxidases, e.g. Myrothecium verrucaria bilirubin oxidase and Rhus vernicifera laccase, respectively, the inflammatory response to HCp in human blood is significantly reduced. Partial purification of HCp allowed to preserve the native conformation of the enzyme and its biocatalytic activity. Therefore, electrochemical studies were carried out with the partially purified enzyme immobilised on nanostructured graphite electrodes at physiological pH and temperature. Amperometric investigations revealed low reductive current densities, i.e. about 1.65 µA cm−2 in oxygenated electrolyte and in the absence of any mediator, demonstrating nevertheless direct electron transfer based O2 bioelectroreduction by HCp for the first time. The reductive current density obtained in the mediated system was about 12 µA cm−2. Even though the inflammatory response of HCp is diminished in human blood, inadequate bioelectrocatalytic performance hinders its use as a cathodic bioelement in a biofuel cell.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Biological fuel cells; Blood; Electrolytes; Electron transport properties; Enzyme activity; Enzyme electrodes; Oxygen; pH; Purification; Redox reactions, Biocatalytic activity; Bioelectronic device; Direct electron transfer; Electrochemical studies; Inflammatory response; Nanostructured graphite; Native conformation; Partial purification, Graphite electrodes
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-42684 (URN)10.1016/j.bioelechem.2021.107794 (DOI)000663599600011 ()33744681 (PubMedID)2-s2.0-85102974379 (Scopus ID)
Available from: 2021-06-03 Created: 2021-06-03 Last updated: 2023-11-29Bibliographically approved
Awad, E., Ramji, R., Cirovic, S., Rämgård, M., Kottorp, A. & Shleev, S. (2021). Developing and evaluating non-invasive healthcare technologies for a group of female participants from a socioeconomically disadvantaged area. Scientific Reports, 11(1), Article ID 23896.
Open this publication in new window or tab >>Developing and evaluating non-invasive healthcare technologies for a group of female participants from a socioeconomically disadvantaged area
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2021 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 11, no 1, article id 23896Article in journal (Refereed) Published
Abstract [en]

When compared to the general population, socioeconomically disadvantaged communitiesfrequently experience compromised health. Monitoring the divide is challenging since standardizedbiomedical tests are linguistically and culturally inappropriate. The aim of this study was to developand test a unique mobile biomedical testbed based on non-invasive analysis, as well as to explorethe relationships between the objective health measures and subjective health outcomes, asevaluated with the World Health Organization Quality of Life survey. The testbed was evaluated in asocioeconomically disadvantaged neighborhood in Malmö, which has been listed as one of the twelvemost vulnerable districts in Sweden. The study revealed that compared to conventional protocolsthe less intrusive biomedical approach was highly appreciated by the participants. Surprisingly, thecollected biomedical data illustrated that the apparent health of the participants from the ethnicallydiverse low-income neighborhood was comparable to the general Swedish population. Statisticallysignificant correlations between perceived health and biomedical data were disclosed, even thoughthe dependences found were complex, and recognition of the manifest complexity needs to beincluded in further research. Our results validate the potential of non-invasive technologies incombination with advanced statistical analysis, especially when combined with linguistically andculturally appropriate healthcare methodologies, allowing participants to appreciate the significanceof the different parameters to evaluate and monitor aspects of health.

Place, publisher, year, edition, pages
Nature Publishing Group, 2021
National Category
Health Care Service and Management, Health Policy and Services and Health Economy
Identifiers
urn:nbn:se:mau:diva-48214 (URN)10.1038/s41598-021-03262-3 (DOI)000729935300085 ()34903797 (PubMedID)2-s2.0-85121044920 (Scopus ID)
Funder
Malmö University, FO 4.3-218/408Malmö University, FO 2020/299Malmö University, FO 2020/299Vinnova, 2017–01272Vinnova, 2016–00421
Available from: 2021-12-16 Created: 2021-12-16 Last updated: 2024-02-05Bibliographically approved
Andoralov, V., Shleev, S., Dergousova, N., Kulikova, O., Popov, V. & Tikhonova, T. (2021). Octaheme nitrite reductase: The mechanism of intramolecular electron transfer and kinetics of nitrite bioelectroreduction.. Bioelectrochemistry, 138, Article ID 107699.
Open this publication in new window or tab >>Octaheme nitrite reductase: The mechanism of intramolecular electron transfer and kinetics of nitrite bioelectroreduction.
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2021 (English)In: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 138, article id 107699Article in journal (Refereed) Published
Abstract [en]

Detailed impedance and voltammetric studies of hexameric octaheme nitrite reductase immobilized on carbon-based nanomaterials, specifically nanotubes and nanoparticles, were performed. Well-pronounced bioelectrocatalytic reduction of nitrite on enzyme-modified electrodes was obtained. Analysis of the impedance data indicated the absence of long-lived intermediates involved in the nitrite reduction. Cyclic voltammograms of biomodified electrodes had a bi-sigmoidal shape, which pointed to the presence of two enzyme orientations on carbon supports. The maximum (limiting) catalytic currents were determined and, by applying the correction by the mixed kinetics equation, the Tafel dependences were plotted for each catalytic wave/each enzyme orientation. Finally, two schemes for the rate-limiting processes during bioelectrocatalysis were proposed, viz. for low- and high-potential orientations.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Bioelectrocatalysis, Intramolecular electron transfer, Octaheme nitrite reductase, Thioalkalivibrio paradoxus
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:mau:diva-37406 (URN)10.1016/j.bioelechem.2020.107699 (DOI)000663533800011 ()33221569 (PubMedID)2-s2.0-85096713287 (Scopus ID)
Available from: 2020-12-08 Created: 2020-12-08 Last updated: 2023-10-31Bibliographically approved
Falk, M., Nilsson, E. J., Cirovic, S., Tudosoiu, B. & Shleev, S. (2021). Wearable Electronic Tongue for Non-Invasive Assessment of Human Sweat. Sensors, 21(21), Article ID 7311.
Open this publication in new window or tab >>Wearable Electronic Tongue for Non-Invasive Assessment of Human Sweat
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2021 (English)In: Sensors, E-ISSN 1424-8220, Vol. 21, no 21, article id 7311Article in journal (Refereed) Published
Abstract [en]

Sweat is a promising biofluid in allowing for non-invasive sampling. Here, we investigate the use of a voltammetric electronic tongue, combining different metal electrodes, for the purpose of non-invasive sample assessment, specifically focusing on sweat. A wearable electronic tongue is presented by incorporating metal electrodes on a flexible circuit board and used to non-invasively monitor sweat on the body. The data obtained from the measurements were treated by multivariate data processing. Using principal component analysis to analyze the data collected by the wearable electronic tongue enabled differentiation of sweat samples of different chemical composition, and when combined with 1H-NMR sample differentiation could be attributed to changing analyte concentrations.

Place, publisher, year, edition, pages
MDPI, 2021
Keywords
electronic tongue, human sweat, non-invasive analysis, wearable sensors
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-46850 (URN)10.3390/s21217311 (DOI)000719077700001 ()34770617 (PubMedID)2-s2.0-85118345498 (Scopus ID)
Available from: 2021-11-15 Created: 2021-11-15 Last updated: 2024-02-05Bibliographically approved
Projects
Wireless electronics powered by nanostructured, flexible, and transparent biofuel cells; Malmö UniversityNon-invasive multi-parameter biomedical devices: Disclosing hidden fitness and health indicators; Malmö UniversityHigh performance cost efficient photoelectric biosupercapacitors reproducibly fabricated with industry-scale throughput; Malmö UniversityCitizens building local resilience by health promotion during the COVID-19 pandemic; Malmö UniversitymHealth in pandemic situations: Smartphone based portable and wearable sensors for COVID-19 diagnostic; Malmö University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-6421-2158

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