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Björklund, SebastianORCID iD iconorcid.org/0000-0001-6254-8539
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Publikasjoner (10 av 39) Visa alla publikasjoner
Labecka, N., Szczepanczyk, M., Mojumdar, E. H., Sparr, E. & Björklund, S. (2024). Unraveling UVB effects: Catalase activity and molecular alterations in the stratum corneum.. Journal of Colloid and Interface Science, 666, 176-188, Article ID S0021-9797(24)00709-4.
Åpne denne publikasjonen i ny fane eller vindu >>Unraveling UVB effects: Catalase activity and molecular alterations in the stratum corneum.
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2024 (engelsk)Inngår i: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 666, s. 176-188, artikkel-id S0021-9797(24)00709-4Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

AIM: Ultraviolet B (UVB) radiation can compromise the functionality of the skin barrier through various mechanisms. We hypothesize that UVB induce photochemical alterations in the components of the outermost layer of the skin, known as the stratum corneum (SC), and modulate its antioxidative defense mechanisms. Catalase is a well-known antioxidative enzyme found in the SC where it acts to scavenge reactive oxygen species. However, a detailed characterization of acute UVB exposure on the activity of native catalase in the SC is lacking. Moreover, the effects of UVB irradiation on the molecular dynamics and organization of the SC keratin and lipid components remain unclear. Thus, the aim of this work is to characterize consequences of UVB exposure on the structural and antioxidative properties of catalase, as well as on the molecular and global properties of the SC matrix surrounding the enzyme.

EXPERIMENTS: The effect of UVB irradiation on the catalase function is investigated by chronoamperometry with a skin covered oxygen electrode, which probes the activity of native catalase in the SC matrix. Circular dichroism is used to explore changes of the catalase secondary structure, and gel electrophoresis is used to detect fragmentation of the enzyme following the UVB exposure. UVB induced alterations of the SC molecular dynamics and structural features of the SC barrier, as well as its water sorption behavior, are investigated by a complementary set of techniques, including natural abundance 13C polarization transfer solid-state NMR, wide-angle X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, and dynamic vapor sorption microbalance.

FINDINGS: The findings show that UVB exposure impairs the antioxidative function of catalase by deactivating both native catalase in the SC matrix and lyophilized catalase. However, UVB radiation does not alter the secondary structure of the catalase nor induce any observable enzyme fragmentation, which otherwise could explain deactivation of its function. NMR measurements on SC samples show a subtle increase in the molecular mobility of the terminal segments of the SC lipids, accompanied by a decrease in the mobility of lipid chain trans-gauche conformers after high doses of UVB exposure. At the same time, the NMR data suggest increased rigidity of the polypeptide backbone of the keratin filaments, while the molecular mobility of amino acid residues in random coil domains of keratin remain unaffected by UVB irradiation. The FTIR data show a consistent decrease in absorbance associated with lipid bond vibrations, relative to the main protein bands. Collectively, the NMR and FTIR data suggest a small modification in the composition of fluid and solid phases of the SC lipid and protein components after UVB exposure, unrelated to the hydration capacity of the SC tissue. To conclude, UVB deactivation of catalase is anticipated to elevate oxidative stress of the SC, which, when coupled with subtle changes in the molecular characteristics of the SC, may compromise the overall skin health and elevate the likelihood of developing skin disorders.

sted, utgiver, år, opplag, sider
Elsevier, 2024
Emneord
Catalase, Keratin filaments, Lipid multilamellar matrix, Oxidative stress, Stratum corneum, UVB, Ultraviolet radiation
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-66988 (URN)10.1016/j.jcis.2024.03.200 (DOI)38593652 (PubMedID)2-s2.0-85189861942 (Scopus ID)
Tilgjengelig fra: 2024-04-26 Laget: 2024-04-26 Sist oppdatert: 2024-04-26bibliografisk kontrollert
Kelly, S. J., Genevskiy, V., Björklund, S., Gonzalez-Martinez, J. F., Poeschke, L., Schröder, M., . . . Kocherbitov, V. (2024). Water Sorption and Structural Properties of Human Airway Mucus in Health and Muco-Obstructive Diseases.. Biomacromolecules, 25(3), 1578-1591
Åpne denne publikasjonen i ny fane eller vindu >>Water Sorption and Structural Properties of Human Airway Mucus in Health and Muco-Obstructive Diseases.
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2024 (engelsk)Inngår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 25, nr 3, s. 1578-1591Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Muco-obstructive diseases change airway mucus properties, impairing mucociliary transport and increasing the likelihood of infections. To investigate the sorption properties and nanostructures of mucus in health and disease, we investigated mucus samples from patients and cell cultures (cc) from healthy, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF) airways. Atomic force microscopy (AFM) revealed mucin monomers with typical barbell structures, where the globule to spacer volume ratio was the highest for CF mucin. Accordingly, synchrotron small-angle X-ray scattering (SAXS) revealed more pronounced scattering from CF mucin globules and suggested shorter carbohydrate side chains in CF mucin and longer side chains in COPD mucin. Quartz crystal microbalance with dissipation (QCM-D) analysis presented water sorption isotherms of the three types of human airway mucus, where, at high relative humidity, COPD mucus had the highest water content compared to cc-CF and healthy airway mucus (HAM). The higher hydration of the COPD mucus is consistent with the observation of longer side chains of the COPD mucins. At low humidity, no dehydration-induced glass transition was observed in healthy and diseased mucus, suggesting mucus remained in a rubbery state. However, in dialyzed cc-HAM, a sorption-desorption hysteresis (typically observed in the glassy state) appeared, suggesting that small molecules present in mucus suppress the glass transition.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2024
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-66269 (URN)10.1021/acs.biomac.3c01170 (DOI)001166555000001 ()38333985 (PubMedID)2-s2.0-85187249840 (Scopus ID)
Tilgjengelig fra: 2024-03-08 Laget: 2024-03-08 Sist oppdatert: 2024-04-11bibliografisk kontrollert
Shafaat, A., Francisco Gonzalez-Martinez, J., O Silva, W., Lesch, A., Nagar, B., Lopes da Silva, Z., . . . Ruzgas, T. (2023). A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.. Angewandte Chemie International Edition, 62(40), Article ID e202308181.
Åpne denne publikasjonen i ny fane eller vindu >>A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.
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2023 (engelsk)Inngår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 62, nr 40, artikkel-id e202308181Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Biofilm-associated infections, which are able to resist antibiotics, pose a significant challenge in clinical treatments. Such infections have been linked to various medical conditions, including chronic wounds and implant-associated infections, making them a major public-health concern. Early-detection of biofilm formation offers significant advantages in mitigating adverse effects caused by biofilms. In this work, we aim to explore the feasibility of employing a novel wireless sensor for tracking both early-stage and matured-biofilms formed by the medically relevant bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The sensor utilizes electrochemical reduction of an AgCl layer bridging two silver legs made by inkjet-printing, forming a part of near-field-communication tag antenna. The antenna is interfaced with a carbon cloth designed to promote the growth of microorganisms, thereby serving as an electron source for reduction of the resistive AgCl into a highly-conductive Ag bridge. The AgCl-Ag transformation significantly alters the impedance of the antenna, facilitating wireless identification of an endpoint caused by microbial growth. To the best of our knowledge, this study for the first time presents the evidence showcasing that electrons released through the actions of bacteria can be harnessed to convert AgCl to Ag, thus enabling the wireless, battery-less, and chip-less early-detection of biofilm formation.

sted, utgiver, år, opplag, sider
John Wiley & Sons, 2023
Emneord
Microbial biofilm, chip-less wireless sensing, inkjet printing, mediated electron transfer, near field communication
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-62039 (URN)10.1002/anie.202308181 (DOI)001090146000021 ()37490019 (PubMedID)2-s2.0-85168699269 (Scopus ID)
Tilgjengelig fra: 2023-08-22 Laget: 2023-08-22 Sist oppdatert: 2024-04-17bibliografisk kontrollert
Morin, M., Björklund, S., Nilsson, E. J. & Engblom, J. (2023). Bicontinuous Cubic Liquid Crystals as Potential Matrices for Non-Invasive Topical Sampling of Low-Molecular-Weight Biomarkers. Pharmaceutics, 15(8), Article ID 2031.
Åpne denne publikasjonen i ny fane eller vindu >>Bicontinuous Cubic Liquid Crystals as Potential Matrices for Non-Invasive Topical Sampling of Low-Molecular-Weight Biomarkers
2023 (engelsk)Inngår i: Pharmaceutics, E-ISSN 1999-4923, Vol. 15, nr 8, artikkel-id 2031Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Many skin disorders, including cancer, have inflammatory components. The non-invasive detection of related biomarkers could therefore be highly valuable for both diagnosis and follow up on the effect of treatment. This study targets the extraction of tryptophan (Trp) and its metabolite kynurenine (Kyn), two compounds associated with several inflammatory skin disorders. We furthermore hypothesize that lipid-based bicontinuous cubic liquid crystals could be efficient extraction matrices. They comprise a large interfacial area separating interconnected polar and apolar domains, allowing them to accommodate solutes with various properties. We concluded, using the extensively studied GMO-water system as test-platform, that the hydrophilic Kyn and Trp favored the cubic phase over water and revealed a preference for locating at the lipid-water interface. The interfacial area per unit volume of the matrix, as well as the incorporation of ionic molecules at the lipid-water interface, can be used to optimize the extraction of solutes with specific physicochemical characteristics. We also observed that the cubic phases formed at rather extreme water activities (>0.9) and that wearing them resulted in efficient hydration and increased permeability of the skin. Evidently, bicontinuous cubic liquid crystals constitute a promising and versatile platform for non-invasive extraction of biomarkers through skin, as well as for transdermal drug delivery.

sted, utgiver, år, opplag, sider
MDPI, 2023
Emneord
tryptophan, kynurenine, tryptophan-to-kynurenine ratio, cancer-related biomarkers, non-invasive extraction, bicontinuous cubic liquid crystal, bilayer partitioning, glycerol monooleate, DOTAP, X-ray diffraction, humidity scanning (HS) QCM-D
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-62643 (URN)10.3390/pharmaceutics15082031 (DOI)001055274500001 ()37631245 (PubMedID)2-s2.0-85168893889 (Scopus ID)
Tilgjengelig fra: 2023-09-18 Laget: 2023-09-18 Sist oppdatert: 2024-07-04bibliografisk kontrollert
Szczepanczyk, M., Paul, L., Ruzgas, T. & Björklund, S. (2023). Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity. Oxygen, 3(1), 77-89
Åpne denne publikasjonen i ny fane eller vindu >>Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity
2023 (engelsk)Inngår i: Oxygen, E-ISSN 2673-9801, Vol. 3, nr 1, s. 77-89Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling consistent fallouts. One major problem, however, is that the activity values obtained with different techniques and procedures can vary to a large extent. The aim of this work was to identify experimental conditions that provide similar catalase activity values with two different methods based on either spectrophotometry or chronoam- perometry. The investigated parameters include the concentration of catalase and its substrate (H2O2), as well as the effect of deoxygenation of the catalase medium by nitrogen (N2). Within the frame of investigated conditions, we show that spectrophotometry is strongly affected by the catalase concen- tration, whereas chronoamperometry is shown to be more dependent on the substrate concentration. Deoxygenation leads to elevated catalase activity values in the case of chronoamperometry, whereas it shows no influence on the results obtained with spectrophotometry. In particular, in the case of low substrate concentrations (i.e., low catalase reaction rates), higher and more accurate results are obtained with deoxygenation in the case of chronoamperometry measurements due to minimized oxygen escape. The effect of deoxygenation, giving rise to elevated catalase activity values, however, is not statistically significant at high substrate concentrations, implying that the protocol can be sim- plified by excluding this step as long as the other parameters are optimized. Finally, by comparing the two methods at different experimental conditions, we identified protocols resulting in similar results, i.e., 10 mM H2O2 and catalase activity of 4–5 U/mL. Based on this work, improved consistency of catalase activity data obtained with different methodologies and in different labs is expected.

sted, utgiver, år, opplag, sider
MDPI, 2023
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-58452 (URN)10.3390/oxygen3010006 (DOI)
Tilgjengelig fra: 2023-03-01 Laget: 2023-03-01 Sist oppdatert: 2023-09-27bibliografisk kontrollert
Morin, M., Runnsjö, A., Ruzgas, T., Engblom, J. & Björklund, S. (2023). Effects of storage conditions on permeability and electrical impedance properties of the skin barrier.. International Journal of Pharmaceutics, 637, 122891, Article ID 122891.
Åpne denne publikasjonen i ny fane eller vindu >>Effects of storage conditions on permeability and electrical impedance properties of the skin barrier.
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2023 (engelsk)Inngår i: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 637, s. 122891-, artikkel-id 122891Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The aim of this study was to investigate the effect of various skin preservation protocols on in vitro drug permeation, epidermal-dermal drug distribution, and electrical impedance properties of skin membranes. Acyclovir (AC) and methyl salicylate (MS) were selected as model drugs due to their different physicochemical properties and skin metabolic profiles. In particular, AC is relatively hydrophilic (logP -1.8) and not expected to be affected by skin metabolism, while MS is relatively lipophilic (logP 2.5) and susceptible to metabolism, being a substrate for esterase residing in skin. Skin from pig ears was used and freshly excised into split-thickness membranes, which were divided and immediately stored at five different storage conditions: a) 4 °C overnight (fresh control), b) 4 °C for 4 days, c) and d) -20 °C for 6 weeks and one year, respectively, and e) -80 °C for 6 weeks. Based on the combined results, general trends are observed showing that fresh skin is associated with lower permeation of both model drugs and higher skin membrane electrical resistance, as compared to the other storage conditions. Interestingly, in the case of fresh skin, significantly lower amounts of MS are detected in the epidermis and dermis compartments, implying higher levels of ester hydrolysis of MS (i.e., higher esterase activity). In line with this, the concentration of salicylic acid (SA) extracted from the dermis is significantly higher for fresh skin, as compared to the other storage conditions. Nevertheless, for all storage conditions, substantial amounts of SA are detected in the receptor medium, as well as in the epidermis and dermis, implying that esterase activity is maintained to some extent in all cases. For AC, which is not expected to be affected by skin metabolism, freeze storage (protocols c-e) is observed to result in higher accumulation of AC in the epidermis, as compared to the case of fresh skin, while the AC concentration in dermis is unaffected. These observations can be rationalized primarily by the observed lower permeability of fresh skin towards this hydrophilic substance. Finally, a strong correlation between AC permeation and electrical skin resistance is shown for individual skin membranes irrespective of storage condition, while the corresponding correlation for MS is inferior. On the other hand, a strong correlation is shown for individual membranes between MS permeation and electrical skin capacitance, while a similar correlation for AC is lower. The observed correlations between drug permeability and electrical impedance open up for standardizing in vitro data for improved analysis and comparisons between permeability results obtained with skin stored at different conditions.

sted, utgiver, år, opplag, sider
Elsevier, 2023
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-59303 (URN)10.1016/j.ijpharm.2023.122891 (DOI)000970186500001 ()36997077 (PubMedID)2-s2.0-85151485213 (Scopus ID)
Tilgjengelig fra: 2023-04-20 Laget: 2023-04-20 Sist oppdatert: 2023-07-04bibliografisk kontrollert
Sparr, E., Björklund, S., Pham, Q. D., Mojumdar, E. H., Stenqvist, B., Gunnarsson, M. & Topgaard, D. (2023). The stratum corneum barrier - From molecular scale to macroscopic properties. Current Opinion in Colloid & Interface Science, 67, Article ID 101725.
Åpne denne publikasjonen i ny fane eller vindu >>The stratum corneum barrier - From molecular scale to macroscopic properties
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2023 (engelsk)Inngår i: Current Opinion in Colloid & Interface Science, ISSN 1359-0294, E-ISSN 1879-0399, Vol. 67, artikkel-id 101725Artikkel, forskningsoversikt (Fagfellevurdert) Published
Abstract [en]

The upper layer of our skin, the stratum corneum (SC), is a versatile material that combines mechanical strength with efficient barrier function. In this paper, we discuss these macroscopic properties of SC in relation to recent findings on molecular responses and structural diversity in SC protein and lipids. We put particular focus on the intermediate (colloidal) length scale and how the different SC substructures are organized with respect to each other, including effects of nonequilibrium conditions in the skin with respect to the gradients in water and other components.

sted, utgiver, år, opplag, sider
Elsevier, 2023
Emneord
Fluid and solid lipids, Keratin filaments, Transport routes, Heterogenous, structures, Skin hydration
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-63088 (URN)10.1016/j.cocis.2023.101725 (DOI)001059987600001 ()2-s2.0-85167567586 (Scopus ID)
Tilgjengelig fra: 2023-10-11 Laget: 2023-10-11 Sist oppdatert: 2023-10-11bibliografisk kontrollert
Shafaat, A., Žalnėravičius, R., Ratautas, D., Dagys, M., Meškys, R., Rutkienė, R., . . . Ruzgas, T. (2022). Glucose-to-Resistor Transduction Integrated into a Radio-Frequency Antenna for Chip-less and Battery-less Wireless Sensing. ACS Sensors, 7(4), 1222-1234
Åpne denne publikasjonen i ny fane eller vindu >>Glucose-to-Resistor Transduction Integrated into a Radio-Frequency Antenna for Chip-less and Battery-less Wireless Sensing
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2022 (engelsk)Inngår i: ACS Sensors, E-ISSN 2379-3694, Vol. 7, nr 4, s. 1222-1234Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

To maximize the potential of 5G infrastructure in healthcare, simple integration of biosensors with wireless tag antennas would be beneficial. This work introduces novel glucose-to-resistor transduction, which enables simple, wireless biosensor design. The biosensor was realized on a near-field communication tag antenna, where a sensing bioanode generated electrical current and electroreduced a nonconducting antenna material into an excellent conductor. For this, a part of the antenna was replaced by a Ag nanoparticle layer oxidized to high-resistance AgCl. The bioanode was based on Au nanoparticle-wired glucose dehydrogenase (GDH). The exposure of the cathode-bioanode to glucose solution resulted in GDH-catalyzed oxidation of glucose at the bioanode with a concomitant reduction of AgCl to highly conducting Ag on the cathode. The AgCl-to-Ag conversion strongly affected the impedance of the antenna circuit, allowing wireless detection of glucose. Mimicking the final application, the proposed wireless biosensor was ultimately evaluated through the measurement of glucose in whole blood, showing good agreement with the values obtained with a commercially available glucometer. This work, for the first time, demonstrates that making a part of the antenna from the AgCl layer allows achieving simple, chip-less, and battery-less wireless sensing of enzyme-catalyzed reduction reaction. 

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2022
Emneord
Internet of Things, wireless detection of glucose, direct electron transfer, glucose dehydrogenase, chip-less wireless sensing
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-51019 (URN)10.1021/acssensors.2c00394 (DOI)000794994500032 ()35392657 (PubMedID)2-s2.0-85128799436 (Scopus ID)
Forskningsfinansiär
Swedish Research Council, 2018-04320Knowledge Foundation, 20170058Knowledge Foundation, 20190010
Tilgjengelig fra: 2022-04-08 Laget: 2022-04-08 Sist oppdatert: 2024-03-05bibliografisk kontrollert
Morin, M., Jankovskaja, S., Ruzgas, T., Henricson, J., Anderson, C. D., Brinte, A., . . . Björklund, S. (2022). Hydrogels and Cubic Liquid Crystals for Non-Invasive Sampling of Low-Molecular-Weight Biomarkers-An Explorative In Vivo Study. Pharmaceutics, 14(2), Article ID 313.
Åpne denne publikasjonen i ny fane eller vindu >>Hydrogels and Cubic Liquid Crystals for Non-Invasive Sampling of Low-Molecular-Weight Biomarkers-An Explorative In Vivo Study
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2022 (engelsk)Inngår i: Pharmaceutics, E-ISSN 1999-4923, Vol. 14, nr 2, artikkel-id 313Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The molecular composition of human skin is altered due to diseases, which can be utilized for non-invasive sampling of biomarkers and disease diagnostics. For this to succeed, it is crucial to identify a sampling formulation with high extraction efficiency and reproducibility. Highly hydrated skin is expected to be optimal for increased diffusion of low-molecular-weight biomarkers, enabling efficient extraction as well as enhanced reproducibility as full hydration represents a well-defined endpoint. Here, the aim was to explore water-based formulations with high water activities, ensuring satisfactory skin hydration, for non-invasive sampling of four analytes that may serve as potential biomarkers, namely tryptophan, tyrosine, phenylalanine, and kynurenine. The included formulations consisted of two hydrogels (chitosan and agarose) and two different liquid crystalline cubic phases based on the polar lipid glycerol monooleate, which were all topically applied for 2 h on 35 healthy subjects in vivo. The skin status of all sampling sites was assessed by electrical impedance spectroscopy and transepidermal water loss, enabling explorative correlations between biophysical properties and analyte abundancies. Taken together, all formulations resulted in the successful and reproducible collection of the investigated biomarkers. Still, the cubic phases had an extraction capacity that was approximately two times higher compared to the hydrogels.

sted, utgiver, år, opplag, sider
MDPI, 2022
Emneord
low-molecular-weight biomarker, tryptophan, kynurenine, tyrosine, phenylalanine, tryptophan-to-kynurenine ratio, skin barrier integrity, stratum corneum, natural moisturizing factor, electrical impedance spectroscopy, transepidermal water loss
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-50952 (URN)10.3390/pharmaceutics14020313 (DOI)000765124800001 ()35214046 (PubMedID)2-s2.0-85124460011 (Scopus ID)
Tilgjengelig fra: 2022-04-06 Laget: 2022-04-06 Sist oppdatert: 2024-07-04bibliografisk kontrollert
Jankovskaja, S., Morin, M., Gustafsson, A., Anderson, C. D., Lehoczki, B., Engblom, J., . . . Ruzgas, T. (2022). Non-Invasive, Topical Sampling of Potential, Low-Molecular Weight, Skin Cancer Biomarkers: A Study on Healthy Volunteers.. Analytical Chemistry, 94(15), 5856-5865
Åpne denne publikasjonen i ny fane eller vindu >>Non-Invasive, Topical Sampling of Potential, Low-Molecular Weight, Skin Cancer Biomarkers: A Study on Healthy Volunteers.
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2022 (engelsk)Inngår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 94, nr 15, s. 5856-5865Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Monitoring of low-molecular weight cancer biomarkers, such as tryptophan (Trp) and its derivative kynurenine (Kyn), might be advantageous to non-invasive skin cancer detection. Thus, we assessed several approaches of topical sampling of Trp and Kyn, in relation to phenylalanine (Phe) and tyrosine (Tyr), on the volar forearm of six healthy volunteers. The sampling was performed with three hydrogels (made of agarose or/and chitosan), hydrated starch films, cotton swabs, and tape stripping. The biomarkers were successfully sampled by all approaches, but the amount of collected Kyn was low, 20 ± 10 pmol/cm2. Kyn quantification was below LOQ, and thus, it was detected only in 20% of topical samples. To mitigate variability problems of absolute amounts of sampled amino acids, Tyr/Trp, Phe/Trp, and Phe/Tyr ratios were assessed, proving reduced inter-individual variation from 79 to 45% and intra-individual variation from 42 to 21%. Strong positive correlation was found between Phe and Trp, pointing to the Phe/Trp ratio (being in the 1.0–2.0 range, at 95% confidence) being least dependent on sampling materials, approaches, and sweating. This study leads to conclusion that due to the difficulty in quantifying less abundant Kyn, and thus the Trp/Kyn ratio, the Phe/Trp ratio might be a possible, alternative biomarker for detecting skin cancers.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2022
HSV kategori
Identifikatorer
urn:nbn:se:mau:diva-51301 (URN)10.1021/acs.analchem.1c05470 (DOI)000792814500018 ()35394278 (PubMedID)2-s2.0-85128387453 (Scopus ID)
Tilgjengelig fra: 2022-05-04 Laget: 2022-05-04 Sist oppdatert: 2023-11-29bibliografisk kontrollert
Prosjekter
Kiselbaserade nanoporösa partiklar för läkemedelsformuleringar; Malmö universitetIcke-invasiv monitorering av hudsjukdomars progression och läkning baserat på lågmolekylära biomarkörer; Malmö universitetLipidnanopartikel – proteininteraktioner: Formuleringsoptimering för bättre terapeutisk effekt; Malmö universitet, Biofilms Research Center for BiointerfacesThe effect of the extracellular lipid organisation on skin barrier function; Malmö universitet, Biofilms Research Center for Biointerfaces
Organisasjoner
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0001-6254-8539