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Sergeeva, Yulia
Publications (2 of 2) Show all publications
Sergeeva, Y., Yeung, S. Y. & Sellergren, B. (2024). Heteromultivalent Ligand Display on Reversible Self-Assembled Monolayers (rSAMs): A Fluidic Platform for Tunable Influenza Virus Recognition. ACS Applied Materials and Interfaces, 16(3), 3139-3146
Open this publication in new window or tab >>Heteromultivalent Ligand Display on Reversible Self-Assembled Monolayers (rSAMs): A Fluidic Platform for Tunable Influenza Virus Recognition
2024 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 16, no 3, p. 3139-3146Article in journal (Refereed) Published
Abstract [en]

We report on the design of heteromultivalent influenza A virus (IAV) receptors based on reversible self-assembled monolayers (SAMs) featuring two distinct mobile ligands. The principal layer building blocks consist of α-(4-amidinophenoxy)alkanes decorated at the ω-position with sialic acid (SA) and the neuraminidase inhibitor Zanamivir (Zan), acting as two mobile ligands binding to the complementary receptors hemagglutinin (HA) and neuraminidase (NA) on the virus surface. From ternary amphiphile mixtures comprising these ligands, the amidines spontaneously self-assemble on top of carboxylic acid-terminated SAMs to form reversible mixed monolayers (rSAMs) that are easily tunable with respect to the ligand ratio. We show that this results in the ability to construct surfaces featuring a very strong affinity for the surface proteins and specific virus subtypes. Hence, an rSAM prepared from solutions containing 15% SA and 10% Zan showed an exceptionally high affinity and selectivity for the avian IAV H7N9 (Kd = 11 fM) that strongly exceeded the affinity for other subtypes (H3N2, H5N1, H1N1). Changing the SA/Zan ratio resulted in changes in the relative preference between the four tested subtypes, suggesting this to be a key parameter for rapid adjustments of both virus affinity and selectivity.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2024
Keywords
H5N1, H7N9, IAV subtype selectivity, virus monitoring, virus recognition
National Category
Biomaterials Science
Identifiers
urn:nbn:se:mau:diva-65698 (URN)10.1021/acsami.3c15699 (DOI)001150615700001 ()38197122 (PubMedID)2-s2.0-85182563097 (Scopus ID)
Available from: 2024-02-02 Created: 2024-02-02 Last updated: 2024-02-27Bibliographically approved
Yeung, S. Y., Sergeeva, Y., Pan, G., Mittler, S., Ederth, T., Dam, T., . . . Sellergren, B. (2022). Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior.. ACS Applied Materials and Interfaces, 14(37), 41790-41799
Open this publication in new window or tab >>Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior.
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2022 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 14, no 37, p. 41790-41799Article in journal (Refereed) Published
Abstract [en]

Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2022
Keywords
ECM mimic, cell modulation, dynamic multivalency, reversible cell adhesion, supported lipid bilayer
National Category
Other Materials Engineering
Identifiers
urn:nbn:se:mau:diva-55183 (URN)10.1021/acsami.2c12029 (DOI)000856045900001 ()36074978 (PubMedID)2-s2.0-85138080666 (Scopus ID)
Available from: 2022-09-27 Created: 2022-09-27 Last updated: 2024-02-05Bibliographically approved
Projects
Dynamic self assembled monolayers as cell membrane mimics and their interactions with cells and pathogensReversible self-assembled monolayers for antibody-free purification and ultrasensitive and rapid in situ detection of viruses; Malmö University, Biofilms Research Center for Biointerfaces
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